Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (Human MCP-4/CCL13) _ C230447

Description

Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13. CCL13 (MCP-4) shares 56-61 % sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60 % identical to Eotaxin (CCL11). CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3.

 

Product Properties

 

Synonyms
CCL13 Protein, Human; CKb10 Protein, Human; MCP-4 Protein, Human; MCP4 Protein, Human; NCC-1 Protein, Human; NCC1 Protein, Human; SCYA13 Protein, Human; SCYL1 Protein, Huma
Accession
Q99616
GeneID
6357

Source

E.coli-derived human Monocyte Chemotactic Protein-4, Gln24-Thr98
Molecular Weight
Approximately 8.6 kDa.
AA Sequence
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.

Endotoxin
Less than 1 EU/μg of the protein as determined by LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.4, 130 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human Monocyte Chemotactic Protein-4/CCL13 (Human MCP-4/CCL13) _ C230447

Product form

SKU: C230447E

$74.00

      • Tell a unique detail about this product

      Description

      Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13. CCL13 (MCP-4) shares 56-61 % sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60 % identical to Eotaxin (CCL11). CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3.

       

      Product Properties

       

      Synonyms
      CCL13 Protein, Human; CKb10 Protein, Human; MCP-4 Protein, Human; MCP4 Protein, Human; NCC-1 Protein, Human; NCC1 Protein, Human; SCYA13 Protein, Human; SCYL1 Protein, Huma
      Accession
      Q99616
      GeneID
      6357

      Source

      E.coli-derived human Monocyte Chemotactic Protein-4, Gln24-Thr98
      Molecular Weight
      Approximately 8.6 kDa.
      AA Sequence
      QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      > 96 % by SDS-PAGE and HPLC analyses.
      Biological Activity

      Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.

      Endotoxin
      Less than 1 EU/μg of the protein as determined by LAL method.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.4, 130 mM NaCl.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
      Further dilutions should be made in appropriate buffered solutions.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Caution

       

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only.

      © 2024 Arcegen, Powered by Shopify

      • American Express
      • Apple Pay
      • Diners Club
      • Discover
      • Meta Pay
      • Google Pay
      • Mastercard
      • Shop Pay
      • Visa

      Login

      Forgot your password?

      Don't have an account yet?
      Create account