Recombinant Human Monocyte Chemotactic Protein-1/CCL2 (Human MCP-1/CCL2)_C230444

Description

CCL2, also called monocyte chemotactic protein- 1 (MCP- 1) or JE, is a member of the C- C or beta chemokine family that is best known as a chemotactic agent for mononuclear  cells . Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a   23 aa signal peptide and a 76 aa mature protein . Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid- modified glutamine, occurs naturally by metalloproteinase cleavage and down- regulates activity but not receptor binding. CCL2 may form multiple bands from 8.7- 13.5 kDa on SDS- PAGE due to non- covalent dimerization and variable carbohydrate content. Mature human CCL2 shares 78- 79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only 56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells.


Product Properties

Synonyms

HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2

Accession

P13500

Source

E.coli-derived Human CCL2 ,Gln23-Thr99.

GeneID

6347

Tag
None
Molecular Weight
Approximately 8.7 kDa.
AA Sequence

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Endotoxin

< 1 EU/μg of protein as determined by LAL method

Purity
> 96%, as determined by SDS-PAGE and HPLC analysis.
Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Human Monocyte Chemotactic Protein-1/CCL2 (Human MCP-1/CCL2)_C230444

Product form

SKU: C230444E

$74.00

    • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    CCL2, also called monocyte chemotactic protein- 1 (MCP- 1) or JE, is a member of the C- C or beta chemokine family that is best known as a chemotactic agent for mononuclear  cells . Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a   23 aa signal peptide and a 76 aa mature protein . Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid- modified glutamine, occurs naturally by metalloproteinase cleavage and down- regulates activity but not receptor binding. CCL2 may form multiple bands from 8.7- 13.5 kDa on SDS- PAGE due to non- covalent dimerization and variable carbohydrate content. Mature human CCL2 shares 78- 79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only 56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells.


    Product Properties

    Synonyms

    HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2

    Accession

    P13500

    Source

    E.coli-derived Human CCL2 ,Gln23-Thr99.

    GeneID

    6347

    Tag
    None
    Molecular Weight
    Approximately 8.7 kDa.
    AA Sequence

    QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

    Endotoxin

    < 1 EU/μg of protein as determined by LAL method

    Purity
    > 96%, as determined by SDS-PAGE and HPLC analysis.
    Biological Activity

    Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only!

    © 2024 Arcegen, Powered by Shopify

    • American Express
    • Apple Pay
    • Diners Club
    • Discover
    • Meta Pay
    • Google Pay
    • Mastercard
    • Shop Pay
    • Visa

    Login

    Forgot your password?

    Don't have an account yet?
    Create account