Description
IGF-1, also known as insulin-like growth factor 1, is a protein that plays a crucial role in growth and development. It is produced mainly by the liver and other tissues in response to growth hormone secretion. IGF-1 is a vital protein in human physiology, contributing to growth, development, and maintenance of various tissues and metabolic processes.
Product Properties
Synonyms
|
IGF-I, IGF1A, somatomedin C, MGF
|
Accession
|
|
Source
|
E.coli-derived Human IGF-1 protein, Gly49-Ala118, with C-terminal Flag tag.
|
Molecular Weight
|
Approximately 8.6 kDa.
|
Tag
|
None
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Purity
|
> 90 % by SDS-PAGE analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 1 ng/ml,corresponding to a specific activity of > 1.0×106 IU/mg.
|
Endotoxin
|
< 1 EU per 1μg of the protein by the LAL method.
|
Formulation
|
Lyophilized from a 0.2 µm filtered solution in PBS.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!