Recombinant Human I-309/CCL1 (Human I-309/CCL1)_C230369

Description

CCL1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. CCL1 interacts with the G protein-linked transmembrane chemokine receptors CCR8 and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that CCL1 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, CCL1 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. CCL1 induces significant levels of LTC4 from elicited eosinophils.

 

Product Properties

Synonyms

Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
Accession
P22362
GeneID
634

Source

E.coli-derived Human I-309/CCL1, Ser23-Lys96.

Molecular Weight

Approximately 8.6 kDa.

AA Sequence

SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL. Fully biologically active when compared to standard.

Endotoxin

1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Human I-309/CCL1 (Human I-309/CCL1)_C230369

Product form

SKU: C230369E

$179.00

      • Tell a unique detail about this product

      Description

      CCL1, also known as I-309 or TCA-3, is a member of the chemokine (C-C motif) ligand family. Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. CCL1 interacts with the G protein-linked transmembrane chemokine receptors CCR8 and induces biochemical events that may result in the control of chemotaxis, proliferation, apoptosis and adhesion. It has been demonstrated that CCL1 displays chemotactic activity for monocytes and other cell types such as NK cells and dendritic cells, but not for neutrophils. Furthermore, as the only known physiological ligand for CCR8, CCL1 was identified as a potent inhibitor of HIV-1 envelope-mediated cell-cell fusion and virus infection. CCL1 induces significant levels of LTC4 from elicited eosinophils.

       

      Product Properties

      Synonyms

      Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
      Accession
      P22362
      GeneID
      634

      Source

      E.coli-derived Human I-309/CCL1, Ser23-Lys96.

      Molecular Weight

      Approximately 8.6 kDa.

      AA Sequence

      SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 97 % by SDS-PAGE and HPLC analyses.

      Biological Activity

      The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL. Fully biologically active when compared to standard.

      Endotoxin

      1 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 100 mM NaCl.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.

      2. For your safety and health, please wear lab coats and disposable gloves for operation.

      3. For research use only!

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