Recombinant Human Growth Regulated Protein-gamma/CXCL3 (Human GRO-γ/CXCL3)_C230365

Description

Human GRO alpha, GRO beta (MIP-2 alpha ), and GRO gamma (MIP-2 beta ) are products of three distinct, non-allelic human genes. GRO beta and GRO gamma share 90% and 86% amino acid sequence homology, respectively, with GRO alpha. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines and are thought to be the homologs of murine KC and MIP-2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

 

Product Properties

Synonyms

GRO-gamma, MIP2-beta
Accession
P19876
GeneID
2921

Source

E.coli-derived Human GRO-γ Ala35-Asn107

Molecular Weight

Approximately 7.9 kDa.

AA Sequence

ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/mL.

Endotoxin

1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Human Growth Regulated Protein-gamma/CXCL3 (Human GRO-γ/CXCL3)_C230365

Product form

SKU: C230365E

$179.00

      • Tell a unique detail about this product

      Description

      Human GRO alpha, GRO beta (MIP-2 alpha ), and GRO gamma (MIP-2 beta ) are products of three distinct, non-allelic human genes. GRO beta and GRO gamma share 90% and 86% amino acid sequence homology, respectively, with GRO alpha. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines and are thought to be the homologs of murine KC and MIP-2. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

       

      Product Properties

      Synonyms

      GRO-gamma, MIP2-beta
      Accession
      P19876
      GeneID
      2921

      Source

      E.coli-derived Human GRO-γ Ala35-Asn107

      Molecular Weight

      Approximately 7.9 kDa.

      AA Sequence

      ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 97 % by SDS-PAGE and HPLC analyses.

      Biological Activity

      Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/mL.

      Endotoxin

      1 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.4, 50 mM NaCl.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.

      2. For your safety and health, please wear lab coats and disposable gloves for operation.

      3. For research use only!

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