Recombinant Human Growth Differentiation Factor 7/Bone Morphogenetic Protein-12 (Human GDF-7/BMP-12)_C230361

Description

Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins. GDF-7 is synthesized as a large precursor protein that consists of an N--terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling  results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8).

 

Product Properties

Synonyms

BMP12GDF7
Accession
Q7Z4P
GeneID
151449

Source

E.coli-derived Human GDF-7/BMP-12, Thr322-Arg450.

Molecular Weight

Approximately 28.0 kDa.

AA Sequence

TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard.

Endotoxin

1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in 30% Acetonitrile and 0.1% TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Human Growth Differentiation Factor 7/Bone Morphogenetic Protein-12 (Human GDF-7/BMP-12)_C230361

Product form

SKU: C230361E

$179.00

      • Tell a unique detail about this product

      Description

      Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins. GDF-7 is synthesized as a large precursor protein that consists of an N--terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling  results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8).

       

      Product Properties

      Synonyms

      BMP12GDF7
      Accession
      Q7Z4P
      GeneID
      151449

      Source

      E.coli-derived Human GDF-7/BMP-12, Thr322-Arg450.

      Molecular Weight

      Approximately 28.0 kDa.

      AA Sequence

      TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 95 % by SDS-PAGE and HPLC analyses.

      Biological Activity

      The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg. Fully biologically active when compared to standard.

      Endotoxin

      1 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 µm filtered solution in 30% Acetonitrile and 0.1% TFA.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.

      2. For your safety and health, please wear lab coats and disposable gloves for operation.

      3. For research use only!

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