Description
G-CSF is a hematopoietic growth factor that stimulates the development of progenitor cells that transform to neutrophils and enhances the functional activity of mature terminal cells.G-CSF is produced in response to specific stimulation of a variety of cells, including macrophages, fibroblasts, endothelial cells, and the bone marrow stroma.G-CSF is used clinically to promote hematopoietic recovery after bone marrow transplantation.G-CSF is used clinically to promote hematopoietic recovery after bone marrow transplantation. This recombinant human G-CSF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.
Product Properties
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Synonyms
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G-CSF, CSF-3, MGI-1G, GM-CSF beta, MGC45931,Pluripoietin
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Accession
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Source
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E.coli-derived Human G-CSF protein, Thr31-Pro204.
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Molecular Weight
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Approximately 18.9 kDa.
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AA Sequence
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TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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The ED50 as determined by a cell proliferation assay using mouse NFS-60 cells is less than 0.05 ng/mL, corresponding to a specific activity of>2.0 x 107 lU/mg.Fully biologically active when compared to standard.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01% Tween-20.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
