Description
GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) is a cytokine that belongs to the colony-stimulating factor family.
It plays an important role in immunomodulation, hematopoiesis and inflammation.GM-CSF is mainly produced by a variety of cells, including macrophages, T cells, B cells and fibroblasts.It is produced by a variety of cells, including macrophages, T cells, B cells and fibroblasts. It can stimulate the proliferation, differentiation and functional activation of granulocytes and monocytes in the hematopoietic cell lineage.GM-CSF can promote the differentiation of pluripotent stem cells in the bone marrow to granulocytes and monocytes, increase the number of leukocytes, and improve their function, thus enhancing the body's immune response and anti-infection ability. In addition, GM-CSF has an important role in regulating the activation of macrophages and dendritic cells and anti-microbial activity. In clinical practice, GM-CSF is widely used in the treatment of bone marrow transplantation-related complications, malignant tumors, and immunodeficiency. It is also used as a tool in laboratory studies to explore the regulatory mechanisms of the hematopoietic and immune systems and to develop new therapeutic strategies.
This recombinant human GM-CSF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.
Product Properties
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Synonyms
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Granulocyte/Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM,
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Accession
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Source
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E.coli-derived human GM-CSF protein, Ala18-Glu144.
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Molecular Weight
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Approximately 14.5 kDa
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AA Sequence
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APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQEPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of>1.0 x 107 lU/mg. Fully biologically active when compared to standard.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
