Recombinant Human GDNF Protein_C230355

Description

GDNF is a disulfide-linked homodimeric neurotrophic factor that is a potent growth factor for dopamine and motor neurons and is structurally related to Artemin, Neurturin, and Persephin.GDNF signals through a multicomponent receptor system consisting of RET and one of the four GFRα (α1-α4) receptors.GDNF specifically promotes dopamine uptake and survival, as well as morphological differentiation of midbrain neurons. In a mouse model of Parkinson's disease, GDNF improves bradykinesia, rigidity and postural instability.This recombinant mouse GDNF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.

 

Product Properties

Synonyms

Astrocyte-derived trophic factor, ATF, ATF-1, ATF2, GDNF, HFB1-GDNF, HGDNF,
HSCR3
Accession
P39905

Source

E.coli-derived Human GDNF protein, Ser78-Ile211.

Molecular Weight

Approximately 31.1 kDa.

AA Sequence

SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV
TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCR
PIAFDDD LSFLDDNLVY HILRKHSAKR CGCI

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml,corresponding to a specific activity of > 5.0×107 IU/mg.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered solution in 1×PBS, pH 7.4, with 0.05 % Tween-20.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Human GDNF Protein_C230355

Product form

SKU: C230355E

$179.00

    • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    GDNF is a disulfide-linked homodimeric neurotrophic factor that is a potent growth factor for dopamine and motor neurons and is structurally related to Artemin, Neurturin, and Persephin.GDNF signals through a multicomponent receptor system consisting of RET and one of the four GFRα (α1-α4) receptors.GDNF specifically promotes dopamine uptake and survival, as well as morphological differentiation of midbrain neurons. In a mouse model of Parkinson's disease, GDNF improves bradykinesia, rigidity and postural instability.This recombinant mouse GDNF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.

     

    Product Properties

    Synonyms

    Astrocyte-derived trophic factor, ATF, ATF-1, ATF2, GDNF, HFB1-GDNF, HGDNF,
    HSCR3
    Accession
    P39905

    Source

    E.coli-derived Human GDNF protein, Ser78-Ile211.

    Molecular Weight

    Approximately 31.1 kDa.

    AA Sequence

    SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV
    TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCR
    PIAFDDD LSFLDDNLVY HILRKHSAKR CGCI

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 97 % by SDS-PAGE and HPLC analyses.

    Biological Activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml,corresponding to a specific activity of > 5.0×107 IU/mg.

    Endotoxin

    < 0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 µm filtered solution in 1×PBS, pH 7.4, with 0.05 % Tween-20.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only!

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