Description
GDNF is a disulfide-linked homodimeric neurotrophic factor that is a potent growth factor for dopamine and motor neurons and is structurally related to Artemin, Neurturin, and Persephin.GDNF signals through a multicomponent receptor system consisting of RET and one of the four GFRα (α1-α4) receptors.GDNF specifically promotes dopamine uptake and survival, as well as morphological differentiation of midbrain neurons. In a mouse model of Parkinson's disease, GDNF improves bradykinesia, rigidity and postural instability.This recombinant mouse GDNF is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.
Product Properties
Synonyms
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Astrocyte-derived trophic factor, ATF, ATF-1, ATF2, GDNF, HFB1-GDNF, HGDNF,
HSCR3
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Accession
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Source
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E.coli-derived Human GDNF protein, Ser78-Ile211.
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Molecular Weight
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Approximately 31.1 kDa.
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AA Sequence
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SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV
TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCR
PIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml,corresponding to a specific activity of > 5.0×107 IU/mg.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in 1×PBS, pH 7.4, with 0.05 % Tween-20.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!