Recombinant Human Fibroblast Growth Factor 23 (Human FGF-23)_C230345

Description

Fibroblast growth factor 23 (FGF- 23) is a member of the FGF family, within a subfamily that also includes FGF- 19 and FGF- 21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta-trefoil structure. β-Klotho has been identified as
co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. Klotho has a restricted distribution that limits FGF- 23 activity. FGF- 23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels. FGF- 23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by down-regulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate.

 

Product Properties

Synonyms

ADHR,HPDR2, HYPF
Accession
Q9GZV9
GeneID
8074
Source
E.coli-derived Human FGF-23, Thr25-Ile227.
Molecular Weight
Approximately 25.3 kDa.
AA Sequence
YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA
FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 × 10 3 IU/mg in the presence of 0.3 μg/mL of rMuKlotho and 10 μg/mL of heparin. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Human Fibroblast Growth Factor 23 (Human FGF-23)_C230345

Product form

SKU: C230345E

$74.00

    • Shipped today? Order within: Oct 04, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    Fibroblast growth factor 23 (FGF- 23) is a member of the FGF family, within a subfamily that also includes FGF- 19 and FGF- 21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta-trefoil structure. β-Klotho has been identified as
    co-factor required for FGF-19, 21, 23 signaling. It can obviously increase ligand-receptor affinity. Klotho has a restricted distribution that limits FGF- 23 activity. FGF- 23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels. FGF- 23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by down-regulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate.

     

    Product Properties

    Synonyms

    ADHR,HPDR2, HYPF
    Accession
    Q9GZV9
    GeneID
    8074
    Source
    E.coli-derived Human FGF-23, Thr25-Ile227.
    Molecular Weight
    Approximately 25.3 kDa.
    AA Sequence
    YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA
    FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 95% by SDS-PAGE and HPLC analyses.
    Biological Activity
    The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 × 10 3 IU/mg in the presence of 0.3 μg/mL of rMuKlotho and 10 μg/mL of heparin. Fully biologically active when compared to standard.
    Endotoxin
    < 1.0 EU per 1μg of the protein by the LAL method.
    Formulation
    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only!

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