Recombinant Human Fibroblast Growth Factor 12 (Human FGF-12)_C230339

Description

Fibroblast growth factor 12 (FGF12) is a fibroblast growth factor homologous factor, a subset of the FGF superfamily. Human FGF-12 is synthesized as a 243 aa protein. In human Vascular smooth muscle cells (VSMCs), FGF12 expression was inhibited at the
transcriptional level by platelet-derived growth factor-BB. FGF12 inhibited cell proliferation through the p53 pathway and upregulated the key factors involved in VSMC lineage differentiation, such as myocardin and serum response factor. In addition,
FGF12 and other FGF11 subfamily members do not activate any fibroblast growth factor receptors (FGFRs), although they can bind to heparin with high affinity like other FGFs. FGF12 has structural similarity with FGF1 and FGF2, in that it lacks a classical signal
sequence and con-tains a nuclear localization signal, resulting in the accu-mulation of FGF12 in the nucleus without any release from cells.

 

Product Properties

Synonyms

FGF12, FGF-12, FGF12B, FHF1, FHF-1, fibroblast growth factor 12, fibroblast growth factor 12
Accession
NP_00410
GeneID
2257
Source
E.coli-derived human FGF-12 protein, Met1-Thr181.
Molecular Weight
Approximately 20.5 kDa.
AA Sequence
MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder
Purity
>98% by SDS-PAGE and HPLC analyses
Biological Activity
The biological activity was determined by its binding ability in a functional ELISA. Immobilized recombinant human FGF R4/Fc Chimera at 5 µg/mL (100 µL/well) can bind recombinant human
FGF-12 with a linear range of 1.6-100 ng/mL.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 1 mM DTT.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Human Fibroblast Growth Factor 12 (Human FGF-12)_C230339

Product form

SKU: C230339E

$179.00

      • Tell a unique detail about this product

      Description

      Fibroblast growth factor 12 (FGF12) is a fibroblast growth factor homologous factor, a subset of the FGF superfamily. Human FGF-12 is synthesized as a 243 aa protein. In human Vascular smooth muscle cells (VSMCs), FGF12 expression was inhibited at the
      transcriptional level by platelet-derived growth factor-BB. FGF12 inhibited cell proliferation through the p53 pathway and upregulated the key factors involved in VSMC lineage differentiation, such as myocardin and serum response factor. In addition,
      FGF12 and other FGF11 subfamily members do not activate any fibroblast growth factor receptors (FGFRs), although they can bind to heparin with high affinity like other FGFs. FGF12 has structural similarity with FGF1 and FGF2, in that it lacks a classical signal
      sequence and con-tains a nuclear localization signal, resulting in the accu-mulation of FGF12 in the nucleus without any release from cells.

       

      Product Properties

      Synonyms

      FGF12, FGF-12, FGF12B, FHF1, FHF-1, fibroblast growth factor 12, fibroblast growth factor 12
      Accession
      NP_00410
      GeneID
      2257
      Source
      E.coli-derived human FGF-12 protein, Met1-Thr181.
      Molecular Weight
      Approximately 20.5 kDa.
      AA Sequence
      MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder
      Purity
      >98% by SDS-PAGE and HPLC analyses
      Biological Activity
      The biological activity was determined by its binding ability in a functional ELISA. Immobilized recombinant human FGF R4/Fc Chimera at 5 µg/mL (100 µL/well) can bind recombinant human
      FGF-12 with a linear range of 1.6-100 ng/mL.
      Endotoxin
      < 0.1 EU per 1μg of the protein by the LAL method
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, with 1 mM DTT.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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