Recombinant Human CXCL12/SDF-1 alpha Protein_C230497

Description

CXCL12, also known as SDF-1, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. They are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. CXCL12 binds primarily to CXC receptor 4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. CXCL12 also can influence lymphopoiesis and regulate patterning and cell number of neural progenitor.

 

Product Properties

 

Synonyms
SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF
Accession
P48061
GeneID
6387

Source

E.coli-derived Human SDF-1α/CXCL12α, Lys22-Lys89
Molecular Weight
Approximately 8.0 kDa.
AA Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE
YLEKALNK
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity
The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/mL. Fully biologically active when
compared to standard.
Endotoxin
Less than 1.0 EU/μg of the protein as determined by LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.

 

Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human CXCL12/SDF-1 alpha Protein_C230497

Product form

SKU: C230497E

$179.00

    • Shipped today? Order within: Apr 26, 2025 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    CXCL12, also known as SDF-1, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. They are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. CXCL12 binds primarily to CXC receptor 4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. CXCL12 also can influence lymphopoiesis and regulate patterning and cell number of neural progenitor.

     

    Product Properties

     

    Synonyms
    SDF-1 alpha, hSDF-1 alpha, IRH, hIRH, PBSF
    Accession
    P48061
    GeneID
    6387

    Source

    E.coli-derived Human SDF-1α/CXCL12α, Lys22-Lys89
    Molecular Weight
    Approximately 8.0 kDa.
    AA Sequence
    KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE
    YLEKALNK
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 97 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/mL. Fully biologically active when
    compared to standard.
    Endotoxin
    Less than 1.0 EU/μg of the protein as determined by LAL method.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
    Further dilutions should be made in appropriate buffered solutions.

     

    Storage

    The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, -20 °C under sterile conditions after reconstitution.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Caution

     

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only.

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