Recombinant Human Cuteaneous T-cell Attracting Chemokine/CCL27 (Human CTACK/CCL27)_C230317

Description

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27. It shares 11% - 35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells . CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to  the skin during inflammation. Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

 

Product Properties

 

Synonyms

CTACK

Source

E.coli-derived Human B cell Activating Factor Rececptor protein,Phe25-Gly112.
AA Sequence
FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG

Endotoxin

< 0.1 EU per μg by the LAL method.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

Dilution

Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

 

Storage

 

 The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1. Please operate with lab coats and disposable gloves,for your safety.       

2. This product is for research use only.  

Recombinant Human Cuteaneous T-cell Attracting Chemokine/CCL27 (Human CTACK/CCL27)_C230317

Product form

SKU: C230317E

$74.00

    • Shipped today? Order within: Feb 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27. It shares 11% - 35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells . CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to  the skin during inflammation. Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

     

    Product Properties

     

    Synonyms

    CTACK

    Source

    E.coli-derived Human B cell Activating Factor Rececptor protein,Phe25-Gly112.
    AA Sequence
    FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG

    Endotoxin

    < 0.1 EU per μg by the LAL method.

    Purity

    > 96% by SDS-PAGE and HPLC analyses.

    Formulation

    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

    Applications

    ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

    Dilution

    Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

     

    Storage

     

     The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Please operate with lab coats and disposable gloves,for your safety.       

    2. This product is for research use only.  

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