Description
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability. Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the
outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Product Properties
|
Synonyms
|
|
|
Source
|
E.coli-derived Human Bcl-2, Ala2-Asp211.
|
|
AA Sequence
|
AHAGRTGYDN REIVMKYIHY KLSQRGYEWD AGDVGAAPPG AAPAPGIFSS QPGHTPHPAA SRDPVARTSP LQTPAAPGAA AGPALSPVPP VVHLTLRQAG DDFSRRYRRD FAEMSSQLHL
TPFTARGRFA TVVEELFRDG VNWGRIVAFF EFGGVMCVES VNREMSPLVD NIALWMTEYL NRHLHTWIQD NGGWDAFVEL YGPSMRPLFD
|
|
Endotoxin
|
< 0.1 EU per μg by the LAL method.
|
|
Purity
|
> 95% by SDS-PAGE and HPLC analyses.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM DTT, 30% Glycerol, with Tween-80.
|
|
Applications
|
ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
|
|
Dilution
|
Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
|
Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
