Recombinant Human Angiostatin (Human Angiostatin)_C230290

Description

Angiostatin is an anti- angiogenic 38- 45 kDa proteolytic fragment of Plasminogen, a 92- 100 kDa glycosylated blood zymogen that serves as the precursor for Plasmin. Plasminogen is produced primarily in the liver, but also in other tissues. Angiostatin circulates in the plasma, binds endothelial cells and myeloid cells, is present in platelet granules, and is excreted in the urine. Human Plasminogen contains an N-terminal activation peptide between amino acids (aa) 1- 98, five characteristically folded kringle domains (aa 103- 561), and a peptidase S1 domain (aa 581- 808). Cleavage of the activation peptide produces mature Plasminogen, while further cleavage between Arg580 and Val581 by tPA (tissue plasminogen activator) produces the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots. Angiostatin was first identified as consisting of kringles 1- 4, a form called K1- 4. Human Angiostatin (K1- 4, aa 99- 459) shares 79- 80% aa sequence identity with mouse, rat, canine, feline, porcine and bovine K1- 4. Other anti- angiogenic forms include kringles 1- 3 (K1- 3), or 1- 4 plus most of kringle 5 (K4.5). K4.5, which is reported to be the most active form, occurs in vivo by autoproteolysis of mature Plasminogen in the presence of either a sulfhydryl donor or cell surface actin, while matrix metalloproteins such as MMP3, 7, 9 and 19 can create multiple forms.

 

Product Properties

 

Synonyms

Angiostatin

Source

E.coli-derived HumanAngiostatin protein,Tyr98-Pro356.

Endotoxin

< 1 EU per μg by the LAL method.

Purity

> 95% by SDS-PAGE  and HPLC analyses.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM NaAc, pH 5.5, 4 % mannitol.

Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

Dilution

Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 

 

Storage

 

 The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1. Please operate with lab coats and disposable gloves,for your safety.       

2. This product is for research use only.  

Recombinant Human Angiostatin (Human Angiostatin)_C230290

Product form

SKU: C230290E

$82.00

    • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    Angiostatin is an anti- angiogenic 38- 45 kDa proteolytic fragment of Plasminogen, a 92- 100 kDa glycosylated blood zymogen that serves as the precursor for Plasmin. Plasminogen is produced primarily in the liver, but also in other tissues. Angiostatin circulates in the plasma, binds endothelial cells and myeloid cells, is present in platelet granules, and is excreted in the urine. Human Plasminogen contains an N-terminal activation peptide between amino acids (aa) 1- 98, five characteristically folded kringle domains (aa 103- 561), and a peptidase S1 domain (aa 581- 808). Cleavage of the activation peptide produces mature Plasminogen, while further cleavage between Arg580 and Val581 by tPA (tissue plasminogen activator) produces the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots. Angiostatin was first identified as consisting of kringles 1- 4, a form called K1- 4. Human Angiostatin (K1- 4, aa 99- 459) shares 79- 80% aa sequence identity with mouse, rat, canine, feline, porcine and bovine K1- 4. Other anti- angiogenic forms include kringles 1- 3 (K1- 3), or 1- 4 plus most of kringle 5 (K4.5). K4.5, which is reported to be the most active form, occurs in vivo by autoproteolysis of mature Plasminogen in the presence of either a sulfhydryl donor or cell surface actin, while matrix metalloproteins such as MMP3, 7, 9 and 19 can create multiple forms.

     

    Product Properties

     

    Synonyms

    Angiostatin

    Source

    E.coli-derived HumanAngiostatin protein,Tyr98-Pro356.

    Endotoxin

    < 1 EU per μg by the LAL method.

    Purity

    > 95% by SDS-PAGE  and HPLC analyses.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM NaAc, pH 5.5, 4 % mannitol.

    Applications

    ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

    Dilution

    Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 

     

    Storage

     

     The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

     

    1. Please operate with lab coats and disposable gloves,for your safety.       

    2. This product is for research use only.  

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