Human CXCL10/IP-10 ELISA Kit_P162021

Description

Human CXCL10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human CXCL10 (Interferon-γ-induced Protein 10) in serum, plasma, and cell culture supernatants. Specific antibodies against Human CXCL10 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the CXCL10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody (biotin-labeled) is added and incubated to bind. Following another wash, an enzyme conjugate (HRP-labeled Streptavidin) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of CXCL10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm (primary wavelength) and 630 nm (secondary wavelength).

IP-10 was initially identified as an IFN-γ-induced gene in monocytes, fibroblasts, and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 determines its membership in the CXC chemokine subfamily.

Specification

Item Number P162021S / P162021E
Specification 48 T / 96 T
Detection Range 15.63-1000 pg/mL
Detection Method Sandwich ELISA
Detection Species Human
Detection Time 4.75 hours
Sensitivity 4.460 pg/mL
Dilution Linearity 72.2 - 119.4%
Recovery Rate 73.3 - 129.6%
Intra-assay Variability 9.2%
Intra-assay Variability 6.5%

Components

Component Number Component Name Storage Temperature P162021S P162021E
P162021-A ELISA Plate 2~8℃ 48 T 96 T
P162021-B Standard 2~8℃ 1 tube 2 tubes
P162021-C Detection Antibody 2~8℃ 1 tube 2 tubes
P162021-D Enzyme Conjugate 2~8℃(Avoid Light) 150 μL 300 μL
P162021-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL
P162021-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
P162021-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
P162021-H Stop Solution Room Temperature 5 mL 10 mL
P162021-I Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After Initial Use

     Material Name Storage Conditions
    Plate  

    Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Human CXCL10/IP-10 ELISA Kit_P162021

    Product form

    SKU: P162021E

    $900.00$285.00

        • Tell a unique detail about this product

        Description

        Human CXCL10 ELISA Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of Human CXCL10 (Interferon-γ-induced Protein 10) in serum, plasma, and cell culture supernatants. Specific antibodies against Human CXCL10 are pre-coated on high-affinity enzyme plates. Standard samples and test samples are added to the wells of the enzyme plate and, after incubation, the CXCL10 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody (biotin-labeled) is added and incubated to bind. Following another wash, an enzyme conjugate (HRP-labeled Streptavidin) is added and incubated to bind. After washing, aTMB chromogenic substrate is added for color development in the absence of light. The intensity of the color reaction is proportional to the concentration of CXCL10 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm (primary wavelength) and 630 nm (secondary wavelength).

        IP-10 was initially identified as an IFN-γ-induced gene in monocytes, fibroblasts, and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 determines its membership in the CXC chemokine subfamily.

        Specification

        Item Number P162021S / P162021E
        Specification 48 T / 96 T
        Detection Range 15.63-1000 pg/mL
        Detection Method Sandwich ELISA
        Detection Species Human
        Detection Time 4.75 hours
        Sensitivity 4.460 pg/mL
        Dilution Linearity 72.2 - 119.4%
        Recovery Rate 73.3 - 129.6%
        Intra-assay Variability 9.2%
        Intra-assay Variability 6.5%

        Components

        Component Number Component Name Storage Temperature P162021S P162021E
        P162021-A ELISA Plate 2~8℃ 48 T 96 T
        P162021-B Standard 2~8℃ 1 tube 2 tubes
        P162021-C Detection Antibody 2~8℃ 1 tube 2 tubes
        P162021-D Enzyme Conjugate 2~8℃(Avoid Light) 150 μL 300 μL
        P162021-E 5x Dilution Buffer 2~8℃ 12 mL 25 mL
        P162021-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
        P162021-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
        P162021-H Stop Solution Room Temperature 5 mL 10 mL
        P162021-I Plate Sealers Room Temperature 3 pieces 5 pieces

        Storage

        The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After Initial Use

           Material Name Storage Conditions
          Plate  

          Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

          Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection Antibody Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
          Stop Solution Can be stored at room temperature.
          Plate Sealers Can be stored at room temperature.

                                            

          Documents:

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