Human IL-21 ELISA Kit_P162019

Description

Human IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-21 in serum, plasma, and cell culture supernatants. Specific anti-IL-21 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-21 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-21 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Human Interleukin-21 (IL-21) is a type I cytokine that regulates the functions of T cells, B cells, NK cells, and myeloid cells. IL-21 is produced by activated follicular helper T cells (Tfh), Th17 cells, and NKT cells. IL-21 derived from Tfh cells plays a crucial role in the development of humoral immunity through its autocrine effects on Tfh cells and paracrine effects on immunoglobulin maturation, plasma cell differentiation, and memory B cells. Mature human IL-21 consists of 133 amino acids and shares 63% and 61% sequence identity with mouse and rat IL-21, respectively. IL-21 is closely associated with clinical conditions such as cancer immunotherapy, viral infections, and allergies. It also inhibits skin hypersensitivity reactions by limiting the production of allergen-specific IgE and degranulation of mast cells.

Specification

Item Number P162019S / P162019E
Specification 48 T / 96 T
Detection Range 15.63-1000 pg/mL
Detection Method Sandwich ELISA
Detection Species Human
Detection Time 4.5 hours
Sensitivity 8.16 pg/mL
Dilution Linearity 81 - 117%
Recovery Rate 76 - 121%
Intra-assay Variability 3.3%
Intra-assay Variability 5.0%

Components

Component Number Component Name Storage Temperature P162019S P162019E
P162019-A ELISA Plate 2~8℃ 48 T 96 T
P162019-B Standard 2~8℃ 1 tube 2 tubes
P162019-C Detection Antibody 2~8℃ 120 μL 240 μL
P162019-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162019-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
P162019-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
P162019-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
P162019-H Stop Solution Room Temperature 5 mL 10 mL
P162019-I Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After Initial Use

     Material Name Storage Conditions
    Plate  

    Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution

    Store at 2~8°C for 1 month, avoiding light exposure.

    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Human IL-21 ELISA Kit_P162019

    Product form

    SKU: P162019E

    $900.00$375.00

        • Tell a unique detail about this product

        Description

        Human IL-21 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-21 in serum, plasma, and cell culture supernatants. Specific anti-IL-21 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-21 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-21 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

        Human Interleukin-21 (IL-21) is a type I cytokine that regulates the functions of T cells, B cells, NK cells, and myeloid cells. IL-21 is produced by activated follicular helper T cells (Tfh), Th17 cells, and NKT cells. IL-21 derived from Tfh cells plays a crucial role in the development of humoral immunity through its autocrine effects on Tfh cells and paracrine effects on immunoglobulin maturation, plasma cell differentiation, and memory B cells. Mature human IL-21 consists of 133 amino acids and shares 63% and 61% sequence identity with mouse and rat IL-21, respectively. IL-21 is closely associated with clinical conditions such as cancer immunotherapy, viral infections, and allergies. It also inhibits skin hypersensitivity reactions by limiting the production of allergen-specific IgE and degranulation of mast cells.

        Specification

        Item Number P162019S / P162019E
        Specification 48 T / 96 T
        Detection Range 15.63-1000 pg/mL
        Detection Method Sandwich ELISA
        Detection Species Human
        Detection Time 4.5 hours
        Sensitivity 8.16 pg/mL
        Dilution Linearity 81 - 117%
        Recovery Rate 76 - 121%
        Intra-assay Variability 3.3%
        Intra-assay Variability 5.0%

        Components

        Component Number Component Name Storage Temperature P162019S P162019E
        P162019-A ELISA Plate 2~8℃ 48 T 96 T
        P162019-B Standard 2~8℃ 1 tube 2 tubes
        P162019-C Detection Antibody 2~8℃ 120 μL 240 μL
        P162019-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
        P162019-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
        P162019-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
        P162019-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
        P162019-H Stop Solution Room Temperature 5 mL 10 mL
        P162019-I Plate Sealers Room Temperature 3 pieces 5 pieces

        Storage

        The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After Initial Use

           Material Name Storage Conditions
          Plate  

          Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

          Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution

          Store at 2~8°C for 1 month, avoiding light exposure.

          Stop Solution Can be stored at room temperature.
          Plate Sealers Can be stored at room temperature.

                                            

          Documents:

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