Human IL-15 ELISA Kit_P162018

Description

Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Human IL-15 in serum, plasma, and cell culture supernatants. Specific anti-IL-15 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-15 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Interleukin-15 (IL-15), a widely expressed 14 kDa cytokine, plays a crucial role in numerous immune-related diseases. It shares approximately 97% and 73% sequence identity with IL-15 from primates and rodents, respectively. IL-15 is secreted by monocytes/macrophages (and some other cells), especially macrophages following viral infection. With similar biological functions to IL-2, IL-15 stimulates T cell activation and proliferation by sharing the γc receptor subunit with IL-2. The roles of IL-15 in autoimmune processes (such as rheumatoid arthritis) and malignancies (such as adult T cell leukemia) underscore its significance as a key immunoregulatory molecule, garnering considerable attention from researchers.

Specification

Item Number P162018S / P162018E
Specification 48 T / 96 T
Detection Range 3.9-250 pg/mL
Detection Method Sandwich ELISA
Detection Species Human
Detection Time 4.5 hours
Sensitivity 1.58 pg/mL
Dilution Linearity 83 - 124%
Recovery Rate 82 - 116%
Intra-assay Variability 3.2%
Intra-assay Variability 5.8%

Components

Component Number Component Name Storage Temperature P162018S P162018E
P162018-A ELISA Plate 2~8℃ 48 T 96 T
P162018-B Standard 2~8℃ 1 tube 2 tubes
P162018-C Detection Antibody 2~8℃ 120 μL 240 μL
P162018-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162018-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
P162018-F

20x Wash Buffer

2~8℃ 25 mL 50 mL
P162018-G

Substrate Solution

2~8℃(Avoid Light) 8 mL 15 mL
P162018-H Stop Solution Room Temperature 5 mL 10 mL
P162018-I Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After First Use

     Material Name Storage Conditions
    Plate  

    Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution

    Store at 2~8°C for 1 month, avoiding light exposure.

    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Human IL-15 ELISA Kit_P162018

    Product form

    SKU: P162018E

    $900.00$375.00

        • Tell a unique detail about this product

        Description

        Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Human IL-15 in serum, plasma, and cell culture supernatants. Specific anti-IL-15 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-15 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-15 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

        Interleukin-15 (IL-15), a widely expressed 14 kDa cytokine, plays a crucial role in numerous immune-related diseases. It shares approximately 97% and 73% sequence identity with IL-15 from primates and rodents, respectively. IL-15 is secreted by monocytes/macrophages (and some other cells), especially macrophages following viral infection. With similar biological functions to IL-2, IL-15 stimulates T cell activation and proliferation by sharing the γc receptor subunit with IL-2. The roles of IL-15 in autoimmune processes (such as rheumatoid arthritis) and malignancies (such as adult T cell leukemia) underscore its significance as a key immunoregulatory molecule, garnering considerable attention from researchers.

        Specification

        Item Number P162018S / P162018E
        Specification 48 T / 96 T
        Detection Range 3.9-250 pg/mL
        Detection Method Sandwich ELISA
        Detection Species Human
        Detection Time 4.5 hours
        Sensitivity 1.58 pg/mL
        Dilution Linearity 83 - 124%
        Recovery Rate 82 - 116%
        Intra-assay Variability 3.2%
        Intra-assay Variability 5.8%

        Components

        Component Number Component Name Storage Temperature P162018S P162018E
        P162018-A ELISA Plate 2~8℃ 48 T 96 T
        P162018-B Standard 2~8℃ 1 tube 2 tubes
        P162018-C Detection Antibody 2~8℃ 120 μL 240 μL
        P162018-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
        P162018-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
        P162018-F

        20x Wash Buffer

        2~8℃ 25 mL 50 mL
        P162018-G

        Substrate Solution

        2~8℃(Avoid Light) 8 mL 15 mL
        P162018-H Stop Solution Room Temperature 5 mL 10 mL
        P162018-I Plate Sealers Room Temperature 3 pieces 5 pieces

        Storage

        The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After First Use

           Material Name Storage Conditions
          Plate  

          Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.

          Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution

          Store at 2~8°C for 1 month, avoiding light exposure.

          Stop Solution Can be stored at room temperature.
          Plate Sealers Can be stored at room temperature.

                                            

          Documents:

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