Human BMP-4 ELISA Kit_P162015

Description

Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

Specification

Item Number P162015S / P162015E
Specification 48 T / 96 T
Detection Range 15.63-1000 pg/mL
Detection Method Sandwich ELISA
Detection Time 4.5 hours
Sensitivity 11.2 pg/mL
Dilution Linearity 86 - 127%
Recovery Rate 75 - 126%
Intra-assay Variability 4.0%
Inter-assay Variability 5.0%

Components

Component Number Component Name Storage Temperature P162015S P162015E
P162015-A Plate 2~8℃ 48 T 96 T
P162015-B Standard 2~8℃ 1 tube 2 tubes
P162015-C Detection Antibody 2~8℃ 60 μL 120 μL
P162015-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162015-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL
P162015-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL
P162015-G 20x Wash Buffer 2~8℃ 25 mL 50 mL
P162015-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
P162015-I Stop Solution Room Temperature 5 mL 10 mL
P162015-J Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After First Use

     Material Name Storage Conditions
    Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Sample Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20x Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution

    Store at 2~8°C for 1 month, avoiding light exposure.

    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Human BMP-4 ELISA Kit_P162015

    Product form

    SKU: P162015E

    $900.00$305.00

        • Tell a unique detail about this product

        Description

        Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

        Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

        Specification

        Item Number P162015S / P162015E
        Specification 48 T / 96 T
        Detection Range 15.63-1000 pg/mL
        Detection Method Sandwich ELISA
        Detection Time 4.5 hours
        Sensitivity 11.2 pg/mL
        Dilution Linearity 86 - 127%
        Recovery Rate 75 - 126%
        Intra-assay Variability 4.0%
        Inter-assay Variability 5.0%

        Components

        Component Number Component Name Storage Temperature P162015S P162015E
        P162015-A Plate 2~8℃ 48 T 96 T
        P162015-B Standard 2~8℃ 1 tube 2 tubes
        P162015-C Detection Antibody 2~8℃ 60 μL 120 μL
        P162015-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
        P162015-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL
        P162015-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL
        P162015-G 20x Wash Buffer 2~8℃ 25 mL 50 mL
        P162015-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
        P162015-I Stop Solution Room Temperature 5 mL 10 mL
        P162015-J Plate Sealers Room Temperature 3 pieces 5 pieces

        Storage

        The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After First Use

           Material Name Storage Conditions
          Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
          Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Sample Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          20x Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution

          Store at 2~8°C for 1 month, avoiding light exposure.

          Stop Solution Can be stored at room temperature.
          Plate Sealers Can be stored at room temperature.

                                            

          Documents:

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