Human BMP-7 ELISA Kit_P162014

Description

Human Bone Morphogenetic Protein-7 (Human BMP-7) is a member of the TGF-β superfamily and is considered one of the most potent synthetic factors for chondrocytes. The growth factor domain of human BMP-7 shares 98% amino acid sequence identity with mouse and rat BMP-7. BMP-7 acts in various organ systems. It promotes new bone formation and the development of renal units, inhibits differentiation of prostate epithelium, and antagonizes epithelial-mesenchymal transition. Bone Morphogenetic Protein (BMP) signaling regulates a series of cellular processes and plays an important role in early embryonic patterning. It is known that BMP-7 expression has a protective effect on renal injury during diabetic nephropathy and disappears in the early stages of diabetic nephropathy progression. Potential clinical applications of BMP-7 include its neuroprotective effects in stroke animal models.

The Arcegen Human BMP-7 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring BMP-7 in serum, plasma, and cell culture supernatants. Specific anti-BMP-7 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. BMP-7 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm).

Specification

Item Number P162014S / P162014E
Specification 48 T / 96 T
Detection Range 62.5-4000 pg/mL
Detection Method Sandwich ELISA
Detection Time 4.5 hours
Sensitivity 44.6 pg/mL
Dilution Linearity 86 - 127%
Recovery Rate 81 - 126%
Intra-assay Variability 3.3%
Inter-assay Variability 4.2%

Components

Component Number Component Name Storage Temperature P162014S P162014E
P162014-A ELISA Plate 2~8℃ 48 T 96 T
P162014-B Standard 2~8℃ 1 tube 2 tubes
P162014-C Detection Antibody 2~8℃ 60 μL 120 μL
P162014-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162014-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL
P162014-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL
P162014-G 20x Wash Buffer 2~8℃ 25 mL 50 mL
P162014-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
P162014-I Stop Solution Room Temperature 5 mL 10 mL
P162014-J Plate Sealant Film Room Temperature 3 pieces 5 pieces

Storage

The kit can be stored at 2~8℃ or according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded, and they should not be reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After First Use

     Component Names Storage Conditions
    ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption.
    Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
    Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
    Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination.
    Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination.
    20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination.
    Substrate Solution

    Store at 2~8°C for 1 month,avoiding light exposure.

    Stop Solution Can be stored at room temperature.
    Plate Sealant Film Can be stored at room temperature.

                                      

    Documents:

    Human BMP-7 ELISA Kit_P162014

    Product form

    SKU: P162014E

    $900.00$325.00

      • Shipped today? Order within: Nov 05, 2024 17:00:00 -0500
      • Tell a unique detail about this product

      Description

      Human Bone Morphogenetic Protein-7 (Human BMP-7) is a member of the TGF-β superfamily and is considered one of the most potent synthetic factors for chondrocytes. The growth factor domain of human BMP-7 shares 98% amino acid sequence identity with mouse and rat BMP-7. BMP-7 acts in various organ systems. It promotes new bone formation and the development of renal units, inhibits differentiation of prostate epithelium, and antagonizes epithelial-mesenchymal transition. Bone Morphogenetic Protein (BMP) signaling regulates a series of cellular processes and plays an important role in early embryonic patterning. It is known that BMP-7 expression has a protective effect on renal injury during diabetic nephropathy and disappears in the early stages of diabetic nephropathy progression. Potential clinical applications of BMP-7 include its neuroprotective effects in stroke animal models.

      The Arcegen Human BMP-7 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitatively measuring BMP-7 in serum, plasma, and cell culture supernatants. Specific anti-BMP-7 antibodies are precoated onto high-affinity enzyme plates. Standard samples and test samples are added to the enzyme plate wells and incubated. BMP-7 in the samples binds to the solid-phase antibodies. After washing away unbound substances, detection antibodies are added and incubated, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation. After washing, TMB substrate solution is added for color development in the dark. The intensity of the color reaction is directly proportional to the concentration of BMP-7 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at a wavelength of 450 nm (with a reference wavelength of 570 - 630 nm).

      Specification

      Item Number P162014S / P162014E
      Specification 48 T / 96 T
      Detection Range 62.5-4000 pg/mL
      Detection Method Sandwich ELISA
      Detection Time 4.5 hours
      Sensitivity 44.6 pg/mL
      Dilution Linearity 86 - 127%
      Recovery Rate 81 - 126%
      Intra-assay Variability 3.3%
      Inter-assay Variability 4.2%

      Components

      Component Number Component Name Storage Temperature P162014S P162014E
      P162014-A ELISA Plate 2~8℃ 48 T 96 T
      P162014-B Standard 2~8℃ 1 tube 2 tubes
      P162014-C Detection Antibody 2~8℃ 60 μL 120 μL
      P162014-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
      P162014-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL
      P162014-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL
      P162014-G 20x Wash Buffer 2~8℃ 25 mL 50 mL
      P162014-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
      P162014-I Stop Solution Room Temperature 5 mL 10 mL
      P162014-J Plate Sealant Film Room Temperature 3 pieces 5 pieces

      Storage

      The kit can be stored at 2~8℃ or according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded, and they should not be reused. The shelf life is 1 year.

      Table 1 Reagent Storage Table After First Use

         Component Names Storage Conditions
        ELISA Plate   Unused strips can be returned to the aluminum foil bag,tightly sealed,and stored at 2-8°C to avoid moisture absorption.
        Standard Use within 48 hours after dissolution,store at 2-8°C to avoid contamination.
        Detection Antibody Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
        Enzyme Conjugate Use within 48 hours after dilution,store at 2~8°C to avoid contamination.
        Sample Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination.
        Antibody/Enzyme Dilution Buffer Store at 2~8°C for 1 month,avoiding contamination.
        20x Wash Buffer Store at 2~8°C for 1 month,avoiding contamination.
        Substrate Solution

        Store at 2~8°C for 1 month,avoiding light exposure.

        Stop Solution Can be stored at room temperature.
        Plate Sealant Film Can be stored at room temperature.

                                          

        Documents:

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