Description
NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility.
Specifications
|
Cat.No.
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N210102S / N210102M
|
|
Size
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24 T / 96 T
|
Components
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Components No.
|
Name
|
N210102S
|
N210102M
|
|
N210102-A
|
2× Frag/Prime Buffe
|
250 μL
|
930 μL
|
|
N210102-B
|
1st Strand Enzyme Mix
|
48 μL
|
192 μL
|
|
N210102-C
|
Strand Specificity Reagent
|
150 μL
|
580 μL
|
|
N210102-D
|
2nd Strand Buffer (dNTP)
|
720 μL
|
2×1440 μL
|
|
N210102-E
|
2nd Strand Buffer (dUTP)
|
720 μL
|
2×1440 μL
|
|
N210102-F
|
2nd Strand Enzyme Master Mix
|
120 μL
|
480 μL
|
|
N210102-G
|
Ligation Enhancer
|
720 μL
|
2×1440 μL
|
|
N210102-H
|
Novel T4 DNA Ligase
|
120 μL
|
480 μL
|
|
N210102-I
|
2 × Super CanaceTM II High-Fidelity Mix
|
600 μL
|
2×1200 μL
|
|
N210102-K
|
Nuclease Free H2O
|
300 μL
|
1000 μL
|
|
N210102-*
|
Primer mix
|
NA
|
NA
|
【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™.
Storage
This product should be stored at -25~-15℃ for 1 year.
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