Description
.Product description
NGS rRNA Depletion Kit (Plant) utilizes RNase H digestion to remove ribosomal RNA (including cytoplasmic 18S, 25S rRNA, mitochondrial 18S, 26S rRNA, and chloroplast 16S, 23S rRNA) from total RNA of plant origin, thereby preserving messenger RNA (mRNA) and other non-coding RNA. This kit provides effective rRNA removal for both intact and partially degraded total RNA samples. The RNA samples obtained after rRNA depletion can be used for high-throughput sequencing to analyze mRNA and non-coding RNA, significantly increasing the proportion of usable sequencing data. They can also be used for cDNA synthesis or other downstream applications.
Application
Suitable for total RNA samples from various plant sources ranging from 100 ng to 1 μg; suitable for both intact and partially degraded RNA samples.
Specifications
|
Cat.No.
|
N210401E / N210401S / N210401M
|
|
Size
|
12 T / 24 T / 96 T
|
Components
|
Components No.
|
Name
|
N210401E
|
N210401S
|
N210401M
|
|
N210401-A
|
Hybridization Buffer
|
36 μL
|
72 μL
|
288 μL
|
|
N210401-B
|
Probe Mix (Plant)
|
36 μL
|
72μL
|
288 μL
|
|
N210401-C
|
RNase H Buffer
|
36 μL
|
72 μL
|
288 μL
|
|
N210401-D
|
Thermostable RNase H
|
24 μL
|
48 μL
|
192 μL
|
|
N210401-E
|
DNase I Buffer
|
330 μL
|
660 μL
|
2×1320 μL
|
|
N210401-F
|
DNase I
|
30 μL
|
60 μL
|
240 μL
|
Storage
Shipped on dry ice, store at -25~-15 °C.Valid for one year.
Notes
1. Please use RNase-free consumables and regularly clean the work area. Thermo Fisher Scientific’s RNAZap™ High-Efficiency Nuclease Removal Spray is recommended for RNase contamination removal.
2. RNA samples should be free of genomic DNA contamination. If gDNA is present, perform DNase I digestion followed by purification before using this kit.
3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the RNA before use.
4. If the RNase H digestion step requires a reaction mix, please prepare it freshly before use.
5. For personal safety and health, wear a lab coat and disposable gloves during operation.
6. This product is for research use only.
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