Description
Product description
NGS rRNA Depletion Kit ( Human/Mouse/Rat) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.
Specifications
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Cat NO.
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N210402E / N210402S / N210402M
|
|
Size
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12 T/24 T /96 T
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Components
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Components No.
|
Name
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N210402E
|
N210402S
|
N210402M
|
|
N210402-A
|
Hybridization Buffer
|
36 μL
|
72 μL
|
288 μL
|
|
N210402-B
|
Human Probe Mix (rRNA & ITS/ETS)
|
24 μL
|
48 μL
|
192 μL
|
|
N210402-C
|
RNase H Buffer
|
36 μL
|
72 μL
|
288 μL
|
|
N210402-D
|
Thermostable RNase H
|
24 μL
|
48 μL
|
192 μL
|
|
N210402-E
|
DNase I Buffer
|
330 μL
|
660 μL
|
2×1320 μL
|
|
N210402-F
|
DNase l
|
30 μL
|
60 μL
|
240 μL
|
Storage
This product should be stored at -25~-15℃ for 1 year.
Notes
1. Use RNase-free consumables and regularly clean work areas. It is recommended to use Thermo Fisher’s RNAZapTM efficient nucleic acid removal spray to eliminate RNase contamination.
2. RNA samples should be free of genomic DNA (gDNA) contamination. If gDNA is present, perform DNase I digestion followed by purification prior to using this kit.
3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the sample before use.
4. If preparing a mix for the RNase H digestion step, prepare it fresh and use immediately.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. This product is intended for research use only!
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