Description
NGS PreMix RNA Library Prep Kit(Strand-specific) is a strand-specific RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility.
Specifications
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Cat.No.
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N210104S / N210104M / N210104MP
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|
Size
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24 T / 96 T / 96 T (plate)
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Components
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Components No.
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Name
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N210104S
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N210104M
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N210104MP**
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N210104-A
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Frag/Primer Buffer
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450 μL
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2×900 μL
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8×266 μL
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N210104-B
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1st Reaction Module 2.0
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192 μL
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768 μL
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8×120 μL
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N210104-C
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2nd Reaction Module(dUTP)
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840 μL
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3×1120 μL
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8×480 μL
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N210104-D
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Ligation Reaction Module
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840 μL
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3×1120 μL
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8×480 μL
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N210104-E
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2×Super CanaceTM II High-Fidelity Mix
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600 μL
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2×1200 μL
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8×340 μL
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*
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Primer Mix*
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/
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/
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/
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[Note]:
* This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately.
This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific primer mixes must be purchased separately:
Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters)
Cat# N210701 — Primer Mix for IlluminaTM
** For the layout of reagents in the pre-loaded plate format, please refer to the figure below.
 |
The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions.
When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment.
It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.
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Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit
Storage
This product should be stored at -25~-15℃ for 1 year.
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