Description
The NGS Flash DNA Library Prep Kit(Enzymatic) is a fast enzymatic library preparation kit. This product utilizes high-quality fragmentation enzymes that integrate the fragmentation with the end repair & A-tailing module, streamlining the workflow and significantly reducing the time and cost of library preparation. It is suitable for input amounts of 100 pg–500 ng of conventional plant and animal genomic DNA, microbial genomic DNA, etc., and allows rapid fragmentation, end repair, and A-tailing reactions to be completed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms.
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Suitable for 100 pg–500 ng genomic DNA samples
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Compatible with IlluminaTM and MGITM high-throughput sequencing platforms
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Fragmentation and end repair completed within 5 minutes
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High library conversion rate and amplification efficiency
Specifications
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Cat.No.
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N210004S / N210001 M
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Size
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24 T / 96 T
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Components
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Components No.
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Name
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N210004E
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N210004S
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N210004-A
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SmearaseTM Mix
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240 μL
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960 μL
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N210004-B
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Ligation Enhancer
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720 μL
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4×720 μL
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N210004-C
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Fast T4 DNA Ligase
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120 μL
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480 μL
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N210004-D
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2×Ultima HF Amplification Mix
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600 μL
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4×600 μL
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*
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Primer Mix*
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120 μL
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480 μL
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【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes:
Cat# N210701 for Illumina™; Cat# N210781 for MGI™.
Storage
This product should be stored at -25~-15℃ for 1 year.
Workflow
Documents：
