Description
NGS DNA Library Prep Kit (Mechanical) is a next-generation library preparation kit specifically developed for IlluminaTM and MGITM high-throughput sequencing platforms. This product utilizes high-quality enzymatic components, improving upon its predecessor by enhancing the efficiency of DNA fragment end repair and A-tailing, as well as optimizing adapter ligation efficiency. The kit employs a novel high-fidelity enzyme, significantly boosting amplification uniformity and fidelity. It can be applied to various samples including routine plant and animal genomes, microbial genomes, FFPE, cfDNA, ChIP DNA, and more, aiding in obtaining excellent sequencing data.
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Applicable to all DNA samples ranging from 100 pg to 1 μg, including cfDNA and FFPE.
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Offers high conversion efficiency, achieving library conversion rates above 70%.
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Verified across multiple samples for excellent library and sequencing outcomes.
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Undergoes stringent batch performance and stability quality control.
Specifications
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Cat.No.
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N210005E / N210005S / N210005M
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Size
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8 T / 24 T / 96 T
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Components
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Components No.
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Name
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N210001E
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N210001S
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N210001M
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N210005-A
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Endprep Buffer 2.0
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56 μL
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168 μL
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672 μL
|
|
N210005-B
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Endprep Enzyme 2.0
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24 μL
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72 μL
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288 μL
|
|
N210005-C
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Ligation Enhancer 2.0
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240 μL
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720 μL
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3×960 μL
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|
N210005-D
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Rapid T4 DNA Ligase 2.0
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80 μL
|
240 μL
|
2×480 μL
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N210005-E
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CanaceTM Pro Amplification Mix
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200 μL
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600 μL
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3×800 μL
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【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes:
Cat# N210701 for Illumina™; Cat# N210781 for MGI™.
Storage
This product should be stored at -25~-15℃ for 1 year.
Workflow
Documents：
