Description
DNA Lib Prep 384 CDI Primer for Illumina is a dedicated adapter primer kit for DNA library preparation on the IlluminaTM high-throughput sequencing platform. It is divided into 2 Sets, each containing PE adapters and 8 types of i5 Index Primers and 12 types of i7 Index Primers used in next-generation sequencing library preparation. The two Sets together provide 16 types of i5 Index Primers and 24 types of i7 Index Primers, which, when used with Arcegen's DNA library prep kits, can construct up to 384 different combinations of dual-indexed libraries. All reagents provided in the kit have undergone strict quality control and functional validation to ensure maximum stability and reproducibility in library preparation.
Specifications
|
Cat.No.
|
N210731S / N210731M
|
|
Size
|
4×2T / 96×2T
|
Components
|
Components No.
|
Name
|
N210731S
|
N210731M
|
|
N210731
|
PE Adapter
|
28 μL
|
2×336 μL
|
|
P501-P502
|
10 μL each
|
-
|
|
P701-P702
|
10 μL each
|
-
|
|
P501-P508
|
-
|
60 μL each
|
|
P701-P712
|
-
|
40 μL each
|
Storage
Store at -25°C ~ -15°C. Valid for 18 months.
Notes
1. The concentration of PE Adapter in this kit is 15 μM, and the amount used per library preparation should be adjusted according to the specific library prep kit used. The concentration of Index Primers is 25 μM.
2. The PE Adapter provided in this kit is a universal short adapter; complete library preparation requires PCR amplification. Index Primers provide Barcode sequence tags for distinguishing samples during high-throughput sequencing.
3. Each Set of this kit contains 8 types of i5 Index Primers (P 5xx) and 12 types of i7 Index Primers (P 7xx), allowing for the preparation of 96 different combinations of dual-indexed libraries. The two Sets together provide 16 types of i5 Index Primers and 24 types of i7 Index Primers. You can use the i5 Index Primers (or i7 Index Primers) from Cat#N210731 in combination with the i7 Index Primers (or i5 Index Primers) from Cat#N210732 to construct up to 384 different combinations of dual-indexed libraries.
4. Do not heat the adapters; allow them to dissolve slowly at room temperature. The laboratory temperature is best maintained between 20–25°C. Avoid repeated freeze-thaw cycles. We recommend aliquoting and storing temporarily at 4°C if needed.
5. The structure of sequencing libraries constructed using the DNA Lib Prep 384 CDI Primer for Illumina kit is as follows:
6. For your safety and health, please wear a lab coat and disposable gloves while handling these reagents.
7. This product is intended solely for research purposes!
Sequence Information
PE Adapter for Illumina:
5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGTC -3´
5´-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3´
i5 Index Primer for Illumina:
5´-AATGATACGGCGACCACCGAGATCTACAC[i5 Index]ACACTCTTTCCCTACACGACGCTCTTCCGATCT -3´
i7 Index Primer for Illumina:
5´-CAAGCAGAAGACGGCATACGAGAT[i7 Index]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-3´
Where [i5 Index] represents an 8 bp i5 Index sequence, and [i7 Index] represents an 8 bp i7 Index sequence. The corresponding Index names, sequences included in the primers, and the Index sequences used during sequencing are shown in the tables below:
|
I5 Index Primers
|
Sequence in Primer
|
Sample Sheet Input / Sequencing Index Sequence
|
|
NovaSeq 6000 v1.0 reagents, MiSeq, HiSeq 2000/2500
|
NovaSeq 6000 v1.5 reagents，
MiniSeq, NextSeq,
HiSeq 3000/4000
|
|
P501
|
TATAGCCT
|
TATAGCCT
|
AGGCTATA
|
|
P502
|
ATAGAGGC
|
ATAGAGGC
|
GCCTCTAT
|
|
P503
|
CCTATCCT
|
CCTATCCT
|
AGGATAGG
|
|
P504
|
GGCTCTGA
|
GGCTCTGA
|
TCAGAGCC
|
|
P505
|
AGGCGAAG
|
AGGCGAAG
|
CTTCGCCT
|
|
P506
|
TAATCTTA
|
TAATCTTA
|
TAAGATTA
|
|
P507
|
CAGGACGT
|
CAGGACGT
|
ACGTCCTG
|
|
P508
|
GTACTGAC
|
GTACTGAC
|
GTCAGTAC
|
|
P509
|
GACCTGTA
|
GACCTGTA
|
TACAGGTC
|
|
P510
|
ATGTAACT
|
ATGTAACT
|
AGTTACAT
|
|
P511
|
GTTTCAGA
|
GTTTCAGA
|
TCTGAAAC
|
|
P512
|
CACAGGAT
|
CACAGGAT
|
ATCCTGTG
|
|
P513
|
TAGCTGCC
|
TAGCTGCC
|
GGCAGCTA
|
|
P514
|
AGCGAATG
|
AGCGAATG
|
CATTCGCT
|
|
P515
|
TATGCTGC
|
TATGCTGC
|
GCAGCATA
|
|
P516
|
AGAAGACT
|
AGAAGACT
|
AGTCTTCT
|
|
I7 Index Primers
|
Sequence in Primer
|
Sample Sheet Input / Sequencing Index Sequence
|
|
P701
|
CGAGTAAT
|
ATTACTCG
|
|
P702
|
TCTCCGGA
|
TCCGGAGA
|
|
P703
|
AATGAGCG
|
CGCTCATT
|
|
P704
|
GGAATCTC
|
GAGATTCC
|
|
P705
|
TTCTGAAT
|
ATTCAGAA
|
|
P706
|
ACGAATTC
|
GAATTCGT
|
|
P707
|
AGCTTCAG
|
CTGAAGCT
|
|
P708
|
GCGCATTA
|
TAATGCGC
|
|
P709
|
CATAGCCG
|
CGGCTATG
|
|
P710
|
TTCGCGGA
|
TCCGCGAA
|
|
P711
|
GCGCGAGA
|
TCTCGCGC
|
|
P712
|
CTATCGCT
|
AGCGATAG
|
|
P713
|
CCTACACG
|
CGTGTAGG
|
|
P714
|
GTAGTGTC
|
GACACTAC
|
|
P715
|
TGTATGCA
|
TGCATACA
|
|
P716
|
CCAGACTG
|
CAGTCTGG
|
|
P717
|
AGGTGCCA
|
TGGCACCT
|
|
P718
|
TCACCTTG
|
CAAGGTGA
|
|
P719
|
GTATCTTT
|
AAAGATAC
|
|
P720
|
CAGCTCCA
|
TGGAGCTG
|
|
P721
|
TCGCCTTA
|
TAAGGCGA
|
|
P722
|
CTAGTACG
|
CGTACTAG
|
|
P723
|
AGCGTAGC
|
GCTACGCT
|
|
P724
|
GAGCCTCG
|
CGAGGCTC
|
Documents:
