Description
Product description
This product contains a DNA molecular weight marker consisting of 6 linear double-stranded DNA fragments at the following sizes: 2,000 bp; 1,000 bp; 750 bp; 500 bp; 250 bp; 100 bp. The reference band is 750 bp, with a concentration of 125 ng/5 μL, while all other bands are at 50 ng/5 μL.
The marker is supplied in 1×DNA Loading Buffer and is designed for agarose gel electrophoresis analysis of DNA bands. It is not recommended for polyacrylamide gel electrophoresis (PAGE).
Specifications
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Product No.
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N132117S
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N132117M
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Size
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100 T
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10×100 T
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Components
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Component No.
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Component Name
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N132117S
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N132117M
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N132117-A
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2 kb DNA Marker
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500 μL
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10×500 μL
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N132117-B
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5×DNA Loading Buffer
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1 mL
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10×1 mL
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Shipping and Storage
Store at room temperatures or at 2°C to 8°C, valid for half a year.
Stor at -25℃ to -15℃, valid for one year. Avoid repeated freeze-thaw cycles.
Notes
1. For optimal electrophoresis results:
1) Ensure thorough mixing of the product before use.
2) Replace the electrophoresis buffer promptly and use freshly prepared gels.
2. If smearing, blurred bands, or distortion occurs during electrophoresis: Dilute the sample with water before loading. For standard-width gel wells, dilute the sample 5-fold with water and load 8-10 μL.
3. When switching to a new stain or using agarose gels containing different stains:
1) Thoroughly clean the electrophoresis tank to avoid cross-contamination.
2) Replace with fresh electrophoresis buffer after cleaning.
4. For your safety and health, please wear a lab coat and disposable gloves.
5. For research use only！
Instructions
1. Load 5 μL of the DNA ladder. For wide wells, increase the loading volume appropriately.
2. Use 1.0-2.0% agarose gels with a voltage of 4–10 V/cm in 0.5×TBE buffer or 1×TAE buffer.
3. Visualize DNA bands under UV light if stain the gel using solution-based staining methods with ethidium bromide (EB) or Arcegen Nucleic Acid Stain (Cat# N132109, non-toxic and UV-compatible).
