Description
Fast RT-gDNA Digestion OneShot SuperMix (for qPCR)
Product description
This product is the brand's highly recommended high-performance cDNA synthesis reagent, featuring faster reverse transcription speed, higher yield, and strong resistance to residual inhibitors. It is suitable for reverse transcription reactions of RNA from animal, plant, and microbial sources. Additionally, the included gDNA Digester Mix can digest up to 1000 ng of DNA, effectively preventing contamination from residual DNA in the RNA template and ensuring more reliable downstream results.
The reverse transcription product of this product can be used for downstream qPCR applications. It is recommended to use in combination with Universal Multiplex qPCR Probe Premix (Cat#N132041), Universal qPCR Dye Premix (Cat#N132031), or Traceable qPCR Dye Premix (Cat#N132034, Cat#N132035, Cat#N132036) for high-performance gene expression analysis.
Specifications
|
Catalog Number
|
N132063E
|
N132063S
|
|
Specifications
|
10 T
|
100 T
|
Components
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Catalog Number
|
Component Name
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N132063E
|
N132063S
|
|
N132063-A
|
4×cDNA OneShot Synthesis Mix
|
50 μL
|
500 μL
|
|
N132063-B
|
gDNA Digester Mix
|
10 μL
|
100 μL
|
|
N132063-C
|
RNase-free H2O
|
1 mL
|
2×1 mL
|
Storage
Store at -25 to -15℃. Valid for 1 year.
Notes
1. All operations should be carried out on ice, and RNase contamination should be avoided during the process.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. For Research Use Only.
Instructions
1. Reaction System Preparation
Thaw Components A, B, and C on ice. Mix each component thoroughly by vortexing. Then, prepare the following reaction system in an RNase-free centrifuge tube:
|
Component
|
Usage Amount
|
|
4×cDNA OneShot Synthesis Mix
|
5 μL
|
|
gDNA Digester Mix
|
1 μL
|
|
Total RNA or mRNA
|
Total RNA：10 pg-5 μg
mRNA：10 pg-500 ng
|
|
RNase-free H2O
|
Up to 20 μL
|
【Note】It is recommended that the input amount of Total RNA does not exceed 2 µg. If the expression level of the target gene is low, the input amount can be increased up to a maximum of 5 µg.
2. Reverse Transcription Protocol Setup
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Temperature
|
Time
|
|
37℃
|
5 min
|
|
85℃
|
30 sec
|
【Notes】
1) This process includes both reverse transcription and gDNA digestion.
2) The reverse transcription product can be directly used for downstream qPCR detection. If the downstream experiment will not be performed immediately, the product can be stored at -20°C. For long-term storage, it is recommended to aliquot and store at -80°C to avoid repeated freeze-thaw cycles.
