Description
The DNA Library Prep Kit for ONT is designed for third-generation Nanopore sequencing platforms. This kit features optimized enzymes and buffers that significantly enhance the adapter ligation efficiency of long fragments. Furthermore, after end-repair and A-tailing, no purification is needed before proceeding directly to barcode ligation, streamlining the workflow.
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Suitable for 100 ng - 1.5 μg gDNA or amplicons.
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Library preparation time is approximately 2 hours.
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Rigorous batch performance and stability quality control measures are in place.
Specifications
|
Cat.No.
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N210007S / N210007M
|
|
Size
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24 T / 96 T
|
Components
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Components No.
|
Name
|
N210007S
|
N210007M
|
|
N210007-A
|
Endprep Buffer
|
168 μL
|
672 μL
|
|
N210007-B
|
Endprep Enzyme
|
72 μL
|
288 μL
|
|
N210007-C
|
Rapid Ligase Master Mix
|
840 μL
|
4×840 μL
|
|
N210007-D
|
Rapid Ligation Reaction Buffer（5×）
|
480 μL
|
2×960 μL
|
|
N210007-E
|
Rapid T4 DNA Ligase
|
120 μL
|
480 μL
|
|
N210007-F
|
Elution Buffer
|
480 μL
|
2×960 μL
|
Storage
This product should be stored at -25~-15℃ for 1 year.
Workflow
