Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free_C231004

Description

Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression.

Arcegel Matrix is a sterile product and free of phenol red with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements.

Feature

  • High Safety: No LDEV (lactate dehydrogenase elevating virus)
  • Multiple Concentration: the concentration range from 8 to 20 mg/ml
  • Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
  • Low Endotoxin: Endotoxin content <8 EU/ml
  • Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
  • High yield:>30L per single batch
  • Wide Compatibility: Compatible with almost all types of cell culture medium

Application

  • 2D Culture Support
  • Migration/Invasion
  • Biochemical Research
  • 3D & Organoid Culture
  • Angiogenesis
  • In Vivo Tumor Formation

Specification

Concentration 8-12 mg/mL
Product Type Basement Membrane Matrix
Endotoxin Level Low
Transportation Conditions Dry Ice Transportation
Product Line Arcegel 
Form Frozen
Phenol Red Indicator None
Product specifications 5/10 mL
Species EHS Mouse Tumors
Classification Basic Type
Serum Level None
LDEV Detection None

Components

Components No. Name C231004E C231004S
C231004 Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free 5 mL 10 mL

Shipping and Storage

Stored at -20°C with a shelf life of 2 years. Transported on dry ice.

Figures

  • Cell application validation

Figure 1. The application validation of Arcegel Matrix were as shown.

The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)

                                 

Figure 2. Intestinal organoids formation

Documents:

Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free_C231004

Product form

SKU: C231004E

$245.00

      • Tell a unique detail about this product

      Description

      Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression.

      Arcegel Matrix is a sterile product and free of phenol red with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements.

      Feature

      • High Safety: No LDEV (lactate dehydrogenase elevating virus)
      • Multiple Concentration: the concentration range from 8 to 20 mg/ml
      • Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
      • Low Endotoxin: Endotoxin content <8 EU/ml
      • Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
      • High yield:>30L per single batch
      • Wide Compatibility: Compatible with almost all types of cell culture medium

      Application

      • 2D Culture Support
      • Migration/Invasion
      • Biochemical Research
      • 3D & Organoid Culture
      • Angiogenesis
      • In Vivo Tumor Formation

      Specification

      Concentration 8-12 mg/mL
      Product Type Basement Membrane Matrix
      Endotoxin Level Low
      Transportation Conditions Dry Ice Transportation
      Product Line Arcegel 
      Form Frozen
      Phenol Red Indicator None
      Product specifications 5/10 mL
      Species EHS Mouse Tumors
      Classification Basic Type
      Serum Level None
      LDEV Detection None

      Components

      Components No. Name C231004E C231004S
      C231004 Arcegel Matrix GFR, Phenol Red-Free, LDEV-Free 5 mL 10 mL

      Shipping and Storage

      Stored at -20°C with a shelf life of 2 years. Transported on dry ice.

      Figures

      • Cell application validation

      Figure 1. The application validation of Arcegel Matrix were as shown.

      The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)

                                       

      Figure 2. Intestinal organoids formation

      Documents:

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