Arcegel Matrix LDEV-Free_C231001

Description

Arcegel Matrix LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components consist of laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression.

Arcegel Matrix is a sterile product with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements, including angiogenesis studies and tumor cell migration.

Feature

  • High Safety: No LDEV (lactate dehydrogenase elevating virus)
  • Multiple Concentration: the concentration range from 8 to 20 mg/ml
  • Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
  • Low Endotoxin: Endotoxin content <8 EU/ml
  • Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
  • High yield:>30L per single batch
  • Wide Compatibility: Compatible with almost all types of cell culture medium

Application

  • 2D Culture Support
  • Migration/Invasion
  • Biochemical Research
  • 3D & Organoid Culture
  • Angiogenesis
  • In Vivo Tumor Formation

Specification

Concentration 8-12 mg/mL
Product Type Basement Membrane Matrix
Endotoxin Level Low
Transportation Conditions Dry Ice Transportation
Product Line Arcegel 
Form Frozen
Phenol Red Indicator Contain
Product specifications 5/10 mL
Species EHS Mouse Tumors
Classification Basic Type
Serum Level None
LDEV Detection None

Components

Components No. Name C231001E C231001S
C231001 Arcegel Matrix LDEV-Free 5 mL 10 mL

Shipping and Storage

Transported on dry ice. Stored at -20°C with a shelf life of 2 years.

Figures

  • Cell application validation

Figure 1. The application validation of Arcegel Matrix were as shown.

The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)

                                 

Figure 2. Intestinal organoids formation

Documents:

Arcegel Matrix LDEV-Free_C231001

Product form

SKU: C231001E

$205.00

    • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    Arcegel Matrix LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components consist of laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), nestin as well as growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors contained in EHS tumors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for studies of cell morphology, biochemical function, migration, invasion and gene expression.

    Arcegel Matrix is a sterile product with a concentration of 8~12 mg/mL, which meets a variety of experimental requirements, including angiogenesis studies and tumor cell migration.

    Feature

    • High Safety: No LDEV (lactate dehydrogenase elevating virus)
    • Multiple Concentration: the concentration range from 8 to 20 mg/ml
    • Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
    • Low Endotoxin: Endotoxin content <8 EU/ml
    • Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
    • High yield:>30L per single batch
    • Wide Compatibility: Compatible with almost all types of cell culture medium

    Application

    • 2D Culture Support
    • Migration/Invasion
    • Biochemical Research
    • 3D & Organoid Culture
    • Angiogenesis
    • In Vivo Tumor Formation

    Specification

    Concentration 8-12 mg/mL
    Product Type Basement Membrane Matrix
    Endotoxin Level Low
    Transportation Conditions Dry Ice Transportation
    Product Line Arcegel 
    Form Frozen
    Phenol Red Indicator Contain
    Product specifications 5/10 mL
    Species EHS Mouse Tumors
    Classification Basic Type
    Serum Level None
    LDEV Detection None

    Components

    Components No. Name C231001E C231001S
    C231001 Arcegel Matrix LDEV-Free 5 mL 10 mL

    Shipping and Storage

    Transported on dry ice. Stored at -20°C with a shelf life of 2 years.

    Figures

    • Cell application validation

    Figure 1. The application validation of Arcegel Matrix were as shown.

    The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)

                                     

    Figure 2. Intestinal organoids formation

    Documents:

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