Description
Recombinant Human Cystine/glutamate transporter (SLC7A11) (Active)
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Name
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Catalog No.
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Size
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Recombinant Human Cystine/glutamate transporter (SLC7A11)
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C230783E
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20 μg
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C230783S
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100 μg
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C230783M
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100 μg
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C230783L
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1 mg
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Product Properties
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Synonyms
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(Amino acid transport system xc-)(Calcium channel blocker resistance protein CCBR1)(Solute carrier family 7 member 11)(xCT)
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Source
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In vitro E.coli expression system
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AA sequence
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Accession # Q9UPY5, Met1 - Leu501
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MW
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58.2KD
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Tag
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N-terminal 10xHis-tagged
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Purity
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>90% as determined by SDS-PAGE.
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Formulation
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Liquid: Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder: Tris/PBS-based buffer, 6% Trehalose.
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Activity
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ELISA: Immobilized Human SLC7A11 at 2 μg/mL can bind Anti-SLC7A11 recombinant antibody, the EC50 is 9.452-13.79 ng/mL.
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Applications
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ELISA, Kinetics (BLI), Kinetics (SPR), Immunization
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Dilution
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Please reconstitute protein in deionized sterile water or PBS to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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Figure
MW and purity analysis by discontinuous SDS-PAGE (reduced).
ELISA analysis of its binding ability. Immobilized Human SLC7A11 at 2μg/mL can bind Anti-SLC7A11 recombinant antibody, the EC50 is 9.452-13.79 ng/mL.
Loaded Anti-Human SLC7A11 Antibody on 96-Flat plate, can bind Human SLC7A11, with an affinity constant of ＜1 pM as determined in BLI assay (Gator Prime).
Storage
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Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Cautions
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
