Recombinant Human Interleukin-36 alpha, 158aa (Human IL-36α, 158aa)_ C230404

Description

Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL- 38 (IL- 1F10) . All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene. IL-36 alpha is an 18- 22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N-linked glycosylation sites. It can be released in response to LPS and the cell ATP-induced activation of the P2X7 receptor. A 120 aa isoform missing aa 1- 38 has been reported. Human IL- 36 alpha (aa 6 - 158) shares 57- 68% aa sequence identity with mouse, rabbit, equine and bovine IL- 36 alpha and 27- 57% aa sequence identity with other novel IL- 1 family members. IL- 36 alpha is mainly found in skin and lymphoid  tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. The receptor for IL- 36 alpha is a combination of IL- 1 Rrp2 (also called IL1RL2 or IL- 1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL- 1 RAcP. IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL- 1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8. IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL- 36 alpha in skin keratinocytes produces epidermal hyperplasia. IL-36 alpha is present in kidney tubule epithelia, and it is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis.

Product Properties

 

Synonyms

FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1

Source

E.coli-derived human IL-36 alpha protein, Met1-Phe158.

AA sequence

MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF

Endotoxin

< 1.0 EU per μg by the LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

Dilution

Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1. Please operate with lab coats and disposable gloves,for your safety.       

2. This product is for research use only.  

Recombinant Human Interleukin-36 alpha, 158aa (Human IL-36α, 158aa)_ C230404

Product form

SKU: C230404E

$179.00

      • Tell a unique detail about this product

      Description

      Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL- 38 (IL- 1F10) . All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene. IL-36 alpha is an 18- 22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N-linked glycosylation sites. It can be released in response to LPS and the cell ATP-induced activation of the P2X7 receptor. A 120 aa isoform missing aa 1- 38 has been reported. Human IL- 36 alpha (aa 6 - 158) shares 57- 68% aa sequence identity with mouse, rabbit, equine and bovine IL- 36 alpha and 27- 57% aa sequence identity with other novel IL- 1 family members. IL- 36 alpha is mainly found in skin and lymphoid  tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. The receptor for IL- 36 alpha is a combination of IL- 1 Rrp2 (also called IL1RL2 or IL- 1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL- 1 RAcP. IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL- 1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8. IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL- 36 alpha in skin keratinocytes produces epidermal hyperplasia. IL-36 alpha is present in kidney tubule epithelia, and it is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis.

      Product Properties

       

      Synonyms

      FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1

      Source

      E.coli-derived human IL-36 alpha protein, Met1-Phe158.

      AA sequence

      MEKALKIDTP QQGSIQDINH RVWVLQDQTL IAVPRKDRMS PVTIALISCR HVETLEKDRG NPIYLGLNGL NLCLMCAKVG DQPTLQLKEK DIMDLYNQPE PVKSFLFYHS QSGRNSTFES VAFPGWFIAV SSEGGCPLIL TQELGKANTT DFGLTMLF

      Endotoxin

      < 1.0 EU per μg by the LAL method.

      Purity

      > 95% by SDS-PAGE and HPLC analyses.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

      Applications

      ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

      Dilution

      Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

       

      1. Please operate with lab coats and disposable gloves,for your safety.       

      2. This product is for research use only.  

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