Recombinant Human IL-22 Protein_ C230399

Description

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT- 1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

 

Product Properties

 

Synonyms

Cytokine Zcyto 18, TIF

Source

E.coli-derived human IL-22 protein, Ala34-Ile179.

AA sequence

MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI 

Endotoxin

< 1.0 EU per μg by the LAL method.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.

Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

Dilution

Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1. Please operate with lab coats and disposable gloves,for your safety.       

2. This product is for research use only.  

Recombinant Human IL-22 Protein_ C230399

Product form

SKU: C230399E

$179.00

      • Tell a unique detail about this product

      Description

      Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT- 1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

       

      Product Properties

       

      Synonyms

      Cytokine Zcyto 18, TIF

      Source

      E.coli-derived human IL-22 protein, Ala34-Ile179.

      AA sequence

      MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI 

      Endotoxin

      < 1.0 EU per μg by the LAL method.

      Purity

      > 97% by SDS-PAGE and HPLC analyses.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.

      Applications

      ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

      Dilution

      Dilute in sterile distilled water or aqueous buffer containing 0.1% BSA.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ for 1 year.

      1 month, 2 to 8 °C under sterile conditions after reconstitution.

      3 months, -20 °C under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

       

      1. Please operate with lab coats and disposable gloves,for your safety.       

      2. This product is for research use only.  

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