Recombinant Human Interleukin-1 Receptor Antagonist Protein (Human IL-1RA)_ C230385

Description

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

 

Product Properties

 

Synonyms

IL-1RN, IRAP

Source

E.coli-derived Human IL-1RA, Arg26-Glu177.

AA sequence

RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF
LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

Endotoxin

< 1.0 EU per μg by the LAL method.

Purity

> 96% by SDS-PAGE and HPLC analyses.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Applications

ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

Dilution

Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.

 

Storage

 

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1. Please operate with lab coats and disposable gloves,for your safety.       

2. This product is for research use only.  

Recombinant Human Interleukin-1 Receptor Antagonist Protein (Human IL-1RA)_ C230385

Product form

SKU: C230385E

$200.00

      • Tell a unique detail about this product

      Description

      IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

       

      Product Properties

       

      Synonyms

      IL-1RN, IRAP

      Source

      E.coli-derived Human IL-1RA, Arg26-Glu177.

      AA sequence

      RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF
      LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE

      Endotoxin

      < 1.0 EU per μg by the LAL method.

      Purity

      > 96% by SDS-PAGE and HPLC analyses.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

      Applications

      ELISA, Kinetics (BLI), Kinetics (SPR), Immunization

      Dilution

      Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

       

      1. Please operate with lab coats and disposable gloves,for your safety.       

      2. This product is for research use only.  

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