Recombinant Human Complement Component C5a Protein _ C230316

Description

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the Cterminal arginine, creating a low activity C5adesArg74 molecule. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.


Product Properties

Synonyms

CPAMD4

Accession

P01031

GeneID

727

Source

E.coli-derived Human C5a, Thr678-Arg751.

Molecular Weight

Approximately 8.3 kDa.

AA Sequence

TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98% by SDS-PAGE and HPLC analyses.

Biological Activity

The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. Fully biologically active when compared to standard. 

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.


Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.


Cautions

  1. Avoid repeated freeze-thaw cycles.
  2. For your safety and health, please wear lab coats and disposable gloves for operation.
  3. For research use only!

Recombinant Human Complement Component C5a Protein _ C230316

Product form

SKU: C230316E

$85.00

      • Tell a unique detail about this product

      Description

      Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain. Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the Cterminal arginine, creating a low activity C5adesArg74 molecule. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.


      Product Properties

      Synonyms

      CPAMD4

      Accession

      P01031

      GeneID

      727

      Source

      E.coli-derived Human C5a, Thr678-Arg751.

      Molecular Weight

      Approximately 8.3 kDa.

      AA Sequence

      TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 98% by SDS-PAGE and HPLC analyses.

      Biological Activity

      The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. Fully biologically active when compared to standard. 

      Endotoxin

      < 1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.


      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

      1 month, 2 to 8 °C under sterile conditions after reconstitution.

      3 months, -20 °C under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.


      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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