Description
Bone morphogenetic protein-4 (BMP-4) is one of nine structurally related BMPs belonging to the transforming growth factor-beta (TGF-beta) superfamily of secreted proteins. The human BMP-4 shares 98 % sequence indentity with mouse BMP-4. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4, a stimulator of chondrogenesis, both in vitro and in vivo, is a potential therapeutic agent for cartilage regeneration.
Product Properties
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Synonyms
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BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4,DVR4, MCOPS6, OFC11, ZYME
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Accession
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P12644
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Source
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E.coli-derived human BMP-4 protein, Ser293-Arg408, E399D.
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Molecular Weight
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The recombinant Human BMP4 predicts a molecular mass of 13.25 kDa.
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Tag
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None
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Purity
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> 95% by SDS-PAGE
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Biological Activity
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Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.08-0.4ug/mL.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from sterile 30% Acetonitrile, 1/1000 TFA. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Avoid repeated freeze-thaw cycles.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only.
