Recombinant Human LIF Protein_C230421

Description

Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine produced by a variety of cell types, including T cells, bone marrow mononuclear lineage, fibroblasts, liver, heart, and melanoma. LIF promotes the long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. Other activities include stimulating the acute-phase protein synthesis in hepatocytes, stimulating the differentiation of cholinergic neurons, and inhibiting lipogenesis in adipocytes by inhibiting lipoprotein lipase. Although human LIF is active on mouse cells and is widely used to maintain mouse ESCs to prevent spontaneous differentiation, mouse LIF is not active on human cells because it cannot bind to the human LIF receptor.

This recombinant human LIF is provided in the form of a lyophilized powder, with high activity, high purity, low endotoxin, and without any additional tags.


Product Properties

Synonyms
CDF, DIA, Differentiation-stimulating Factor, D Factor, Emfilermin, HILDA, Melanoma-derived LPL Inhibitor, MLPLI
Source
E.coli-derived human LIF, Ser23-Phe202.
Accession
P15018
Tag
None
Molecular Weight
Approximately 19.7 kDa.
AA Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Endotoxin
< 1.0 EU/μg by the LAL method.
Purity
> 98%, as determined by SDS-PAGE and HPLC analysis.
Biological Activity
The ED5adeterminebthdose-dependenproliferatioof human TF-cells iless tha0.ng/mLcorrespondintspecifiactivito1.× 10 ^7
 IU/mgFully biologically active when compared to standard.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance
White lyophilized powder

 

Shipping and Storage

Store at -25 to -15°C, with a shelf life of 1 year from the date of receipt of goods.
After reconstitution, store at 2 to 8°C, with a shelf life of 7 days.
After reconstitution, store at -85 to -65°C, with a shelf life of 3 months.

 

Reconstitution Method

Before opening, centrifuge briefly to bring the contents to the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL, which can be further diluted according to subsequent experimental needs; for long-term storage, it is recommended to include a carrier protein (0.1% BSA) in the dilution buffer; aliquot for a single experimental use and store at -80°C to avoid repeated freeze-thaw cycles.

Cautions

1. For your safety and health, please wear a lab coat and use disposable gloves when handling.
2. This product is for research purposes only.

Recombinant Human LIF Protein_C230421

Product form

SKU: C230421E

$74.00

      • Tell a unique detail about this product

      Description

      Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine produced by a variety of cell types, including T cells, bone marrow mononuclear lineage, fibroblasts, liver, heart, and melanoma. LIF promotes the long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. Other activities include stimulating the acute-phase protein synthesis in hepatocytes, stimulating the differentiation of cholinergic neurons, and inhibiting lipogenesis in adipocytes by inhibiting lipoprotein lipase. Although human LIF is active on mouse cells and is widely used to maintain mouse ESCs to prevent spontaneous differentiation, mouse LIF is not active on human cells because it cannot bind to the human LIF receptor.

      This recombinant human LIF is provided in the form of a lyophilized powder, with high activity, high purity, low endotoxin, and without any additional tags.


      Product Properties

      Synonyms
      CDF, DIA, Differentiation-stimulating Factor, D Factor, Emfilermin, HILDA, Melanoma-derived LPL Inhibitor, MLPLI
      Source
      E.coli-derived human LIF, Ser23-Phe202.
      Accession
      P15018
      Tag
      None
      Molecular Weight
      Approximately 19.7 kDa.
      AA Sequence
      SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
      Endotoxin
      < 1.0 EU/μg by the LAL method.
      Purity
      > 98%, as determined by SDS-PAGE and HPLC analysis.
      Biological Activity
      The ED5adeterminebthdose-dependenproliferatioof human TF-cells iless tha0.ng/mLcorrespondintspecifiactivito1.× 10 ^7
       IU/mgFully biologically active when compared to standard.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
      Physical Appearance
      White lyophilized powder

       

      Shipping and Storage

      Store at -25 to -15°C, with a shelf life of 1 year from the date of receipt of goods.
      After reconstitution, store at 2 to 8°C, with a shelf life of 7 days.
      After reconstitution, store at -85 to -65°C, with a shelf life of 3 months.

       

      Reconstitution Method

      Before opening, centrifuge briefly to bring the contents to the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL, which can be further diluted according to subsequent experimental needs; for long-term storage, it is recommended to include a carrier protein (0.1% BSA) in the dilution buffer; aliquot for a single experimental use and store at -80°C to avoid repeated freeze-thaw cycles.

      Cautions

      1. For your safety and health, please wear a lab coat and use disposable gloves when handling.
      2. This product is for research purposes only.

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