Recombinant Human Flt-3 Ligand/FLT3L Protein_C230348

Description

The cytokine Fms-like tyrosine kinase 3 ligand (FLT3L), also known as Flt-3 Ligand, is an important regulator of hematopoiesis. Its receptor, Flt3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and
differentiation factor for several hematopoietic lineages. Activating mutations of Flt3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Mature human Flt- 3L consists of a 158 amino acid (aa) extracellular
domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins
exhibit cross-species activity. The Flt3-L manifests antitumor activity, presumably due to its capacity to recruit dendritic cells and
cause their proliferation.

 

Product Properties

Synonyms

Flt3-Ligand, FL, FLG3L, Flt3L, SL Cytokin
Accession
P49771
GeneID
2323
Source
E.coli-derived human IL-1alpha protein, Thr27-Ala181.
Molecular Weight
Approximately 17.6 kDa.
AA Sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT
RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10 6 IU/mg. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human Flt-3 Ligand/FLT3L Protein_C230348

Product form

SKU: C230348E

$155.00

      • Tell a unique detail about this product

      Description

      The cytokine Fms-like tyrosine kinase 3 ligand (FLT3L), also known as Flt-3 Ligand, is an important regulator of hematopoiesis. Its receptor, Flt3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and
      differentiation factor for several hematopoietic lineages. Activating mutations of Flt3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Mature human Flt- 3L consists of a 158 amino acid (aa) extracellular
      domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins
      exhibit cross-species activity. The Flt3-L manifests antitumor activity, presumably due to its capacity to recruit dendritic cells and
      cause their proliferation.

       

      Product Properties

      Synonyms

      Flt3-Ligand, FL, FLG3L, Flt3L, SL Cytokin
      Accession
      P49771
      GeneID
      2323
      Source
      E.coli-derived human IL-1alpha protein, Thr27-Ala181.
      Molecular Weight
      Approximately 17.6 kDa.
      AA Sequence
      TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT
      RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      > 97% by SDS-PAGE and HPLC analyses.
      Biological Activity
      The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10 6 IU/mg. Fully biologically active when compared to standard.
      Endotoxin
      < 1.0 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ for 1 year.
      1 month, 2 to 8 °C under sterile conditions after reconstitution.
      3 months, -20 °C under sterile conditions after reconstitution.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only.

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