Arcegel Matrix Gel Application - Matrix Gel Plug Assay
Angiogenesis is closely associated with wound healing, inflammation, rheumatoid arthritis, diabetic retinopathy, macular degeneration, and tumor growth. During the growth process of organisms, angiogenesis is an important component of new tissue formation. In adulthood, apart from periodic events tightly regulated in specific organs, nearly every normal tissue lacks substantial physiological angiogenesis due to the balance between pro-angiogenic and anti-angiogenic endogenous factors. When the balance toward pro-angiogenesis is disrupted, microvascular endothelial cells (ECs) can adopt an angiogenic phenotype, initiating angiogenic responses that can be either eliminated or accelerated. Traditional in vivo angiogenesis experimental methods involved in research include corneal micropocket, mesenteric, sponge/matrix implants, disk analysis system (DAS), and zebrafish.
The most commonly used matrix for maintaining cell growth in model generation is the soluble basement membrane preparation extracted from EHS mouse tumors, known as matrix gel, to ensure the success of angiogenesis or plug assays. Arcegel Matrix Gel is free of LDEV (lactic dehydrogenase elevating virus), has an ultra-low endotoxin content, and has undergone testing to guarantee absence of mycoplasma contamination, including different types of matrix gel such as basic concentration, high concentration, and low growth factor concentration.
Application directions
Product features
High Safety: Free of LDEV (Lactic Dehydrogenase Elevating Virus);
Diverse Concentrations: Concentration ranges between 8~20 mg/mL;
Good Batch Stability: Strict production quality inspection process ensures stable performance between batches;
Low Endotoxin: Endotoxin content <8 EU/mL;
Contaminant Detection: Tested negative for mycoplasma, bacteria, and fungal residues;
High Yield: Production exceeds 50L/month;
Excellent Compatibility: Compatible with any type of cell culture medium.
Matrix Gel Plug Assay
Experimental Procedure
1. Place BALB/c nude mice aged 3-7 weeks in a laminar flow barrier system for housing.
2. Remove Arcegel Matrix Gel from the -20°C freezer and allow it to thaw at 4°C for 12 hours until it becomes liquid.
3. Divide the mice into experimental groups: control group, VEGFA group, heparin sodium group, and VEGFA+heparin sodium group.
4. For the control group, inject 0.5 mL of high-concentration Arcegel Matrix Gel subcutaneously into the nude mice. For the VEGFA and heparin sodium groups, inject 0.5 mL of Matrix Gel containing VEGFA (20 μg/L) or heparin sodium (50 U/mL) subcutaneously into the nude mice, respectively. For the VEGFA+heparin sodium group, inject 0.5 mL of Matrix Gel containing a mixture of VEGFA (20 μg/L) and heparin sodium (50 U/mL) subcutaneously into the nude mice. The subcutaneous injection site should be near the midline of the mouse abdomen.
5. After 1 week, euthanize the nude mice and remove the matrix gel plugs. Measure hemoglobin concentration strictly according to the Drabkin reagent method.
Note: Preparation of Drabkin reagent: Dissolve 1.0g of sodium bicarbonate, 0.05g of potassium hydrogen phosphate, and 0.2g of ferric chloride in distilled water to a total volume of 1,000 mL. Store at 4°C.
Experimental Results
Figure: Results of BALB/c nude mice cultured for 7 days
FAQ
What considerations should be taken into account during experimental procedures?
1. The high-concentration matrix gel tends to solidify easily. Pre-cool the matrix gel and injection needles on ice before inoculation.
2. Subcutaneous injection volume in nude mice is relatively large. Ensure proper needle depth to prevent leakage.
3. Maintain normal feeding and observe the injection site of the matrix gel. Stability can be observed for 1-2 weeks.
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