Rapid Cell Proliferation Tracing Assay
CFDA, SE is membrane-permeable and does not exhibit intrinsic fluorescence. Upon passive transport across the cell membrane into viable cells, it is catalyzed by intracellular esterases to generate carboxyfluorescein succinimidyl ester (CFSE), which exhibits strong green fluorescence and is retained within the cells. CFSE can also spontaneously and irreversibly bind to intracellular amines, thereby conjugating to cellular proteins. Excess and unbound CFDA, SE diffuse passively back into the extracellular culture medium and are removed by subsequent washing steps. The fluorescence of non-dividing cells labeled with CFDA, SE is highly stable, lasting for several months, making it particularly suitable for cell population analysis.
The fluorescence of CFDA, SE-labeled cells is very homogeneous, superior to previous fluorescent cell tracing probes such as PKH26, and the fluorescence distribution of daughter cells after division is more uniform. During cell division and proliferation, CFSE-labeled fluorescence is evenly distributed into two daughter cells, with the fluorescence intensity halved compared to the parent cell. Using a flow cytometer (FL1 channel), undivided cells, as well as cells that have undergone one (1/2 fluorescence intensity), two (1/4 fluorescence intensity), three (1/8 fluorescence intensity), or more divisions can be detected based on the different fluorescence intensities. CFSA, SE can detect cell divisions up to eight times or even more. Cells labeled with CFDA, SE can be used for both in vitro and in vivo proliferation studies and do not stain adjacent cells. CFDA, SE is commonly used for proliferation detection in lymphocytes and can also be used for fibroblasts, natural killer cells, hematopoietic progenitor cells, and other cell proliferation studies.
CFDA, SE-labeled cells exhibit green fluorescence with Ex=494nm and Em=521nm. In addition to flow cytometry for detecting cell proliferation, fluorescent enzyme-linked immunosorbent assay (ELISA) can be used for quantitative determination of viable cell numbers, or fluorescent microscopy can be used for homogeneous staining and cell tracing observation.
Furthermore, a CFDA, SE Cell Proliferation and Tracing Assay Kit can be provided, which includes solvents required for preparing CFDA, SE storage solution and staining buffer for cell labeling, simplifying the experimental preparation work.
|
Catalog Number |
Product Name |
Specification |
|
C331306E |
CFDA SE Cell Proliferation and Tracking Kit |
500T |
|
C331306S |
CFDA SE Cell Proliferation and Tracking Kit |
1000T |
|
C331307E |
CFDA SE Cell Proliferation and Tracking Probe |
25mg |