Recombinant Human soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B (Human sTNF RII/TNFRSF1B)_C230491

Description

TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

Product Properties

 

Synonyms
Human sTNF RII
Accession
P20333
GeneID
7133

Source

E.coli-derived Human sTNF RI protein,Ile22-Thr211.
Molecular Weight
Approximately 20.0kDa.
AA Sequence
MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST
YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK
CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/mL, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.

Endotoxin
Less than 0.1 EU/μg of the protein as determined by LAL method.
Formulation
Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human soluble Tumor Necrosis Factor Receptor Type II/TNFRSF1B (Human sTNF RII/TNFRSF1B)_C230491

Product form

SKU: C230491E

$74.00

    • Shipped today? Order within: Feb 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

    Product Properties

     

    Synonyms
    Human sTNF RII
    Accession
    P20333
    GeneID
    7133

    Source

    E.coli-derived Human sTNF RI protein,Ile22-Thr211.
    Molecular Weight
    Approximately 20.0kDa.
    AA Sequence
    MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST
    YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK
    CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 97 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 0.2 μg/mL, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-α.

    Endotoxin
    Less than 0.1 EU/μg of the protein as determined by LAL method.
    Formulation
    Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
    Further dilutions should be made in appropriate buffered solutions.

     

    Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Caution

     

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only.

    © 2025 Arcegen, Powered by Shopify

    • American Express
    • Apple Pay
    • Diners Club
    • Discover
    • Google Pay
    • Mastercard
    • Shop Pay
    • Visa

    Login

    Forgot your password?

    Don't have an account yet?
    Create account