CleanRNA™ T7 RNA Polymerase(250U/μL)_N120002

Description

The product is a T7 RNA polymerase derived from recombinant expression of Escherichia coli, which is a modified and optimized mutant of the wild-type T7 that can significantly reduce the content of dsRNA. It uses a double-stranded DNA containing a T7 promoter sequence as a template, with NTPs as substrates, to synthesize RNA that is complementary to the single-stranded DNA downstream of the promoter and opposite to it. Both double-stranded linear blunt ends and 5' overhanging ends DNA can serve as substrate templates for T7 RNA polymerase, thus linear plasmids and PCR products can all be used as templates for in vitro RNA synthesis.

 

Specifications

Source

Recombinant E.coli 

Optimum Temperature

37℃

Storage Buffer

50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100, 50% (v/v) glycerol, pH 7.9 at 25°C.

Unit Definition

Under conditions of 37°C and pH 8.0, the amount of enzyme required to incorporate 1 nmol of [3H]GMP into acid-insoluble precipitate within 1 hour is defined as one unit of activity.


Shipping and Storage

Shipped on dry ice. Store at -15°C~ -25°C for one years.



CleanRNA™ T7 RNA Polymerase(250U/μL)_N120002

Product form

SKU: N120002E

$348.00

    • Tell a unique detail about this product

    Description

    The product is a T7 RNA polymerase derived from recombinant expression of Escherichia coli, which is a modified and optimized mutant of the wild-type T7 that can significantly reduce the content of dsRNA. It uses a double-stranded DNA containing a T7 promoter sequence as a template, with NTPs as substrates, to synthesize RNA that is complementary to the single-stranded DNA downstream of the promoter and opposite to it. Both double-stranded linear blunt ends and 5' overhanging ends DNA can serve as substrate templates for T7 RNA polymerase, thus linear plasmids and PCR products can all be used as templates for in vitro RNA synthesis.

     

    Specifications

    Source

    Recombinant E.coli 

    Optimum Temperature

    37℃

    Storage Buffer

    50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100, 50% (v/v) glycerol, pH 7.9 at 25°C.

    Unit Definition

    Under conditions of 37°C and pH 8.0, the amount of enzyme required to incorporate 1 nmol of [3H]GMP into acid-insoluble precipitate within 1 hour is defined as one unit of activity.


    Shipping and Storage

    Shipped on dry ice. Store at -15°C~ -25°C for one years.



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