DNase I (2 U/μL)_N120005

Description

Product description

DNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones).The optimum pH range is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions, such as Co2+, Mn2+, Zn2+, etc. 5 mM Ca2+ can protect the enzyme from being hydrolyzed. In the presence of Mg2+, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn2+, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.

 

Specifications

Source
Recombinant E. coli with DNase I gene.
Optimum Temperature
37℃
Storage Buffer
10 mM Tris-HCl pH 7.62 mM CaCl250%(v/v) Glycerol.
Unit Definition
The amount of enzyme required to increase the absorbance at 260 nm of the reaction solution by 0.001 in 1 minute at 25°C and pH 5.0 using calf thymus DNA as the substrate is defined as one activity unit (Kunitz Unit).(The reaction buffer is: 10 mM Tris-HCl pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 μg plasmid DNA.)


Shipping and Storage

Shipped on dry ice. Store at -15°C~ -25°C for one years.



DNase I (2 U/μL)_N120005

Product form

SKU: N120005E

$98.00

    • Tell a unique detail about this product

    Description

    Product description

    DNase I is an endonuclease that can digest single-stranded and double-stranded DNA to produce single deoxynucleotides or single stranded or double-stranded oligodeoxynucleotides. It can hydrolyze the phosphodiester bond to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The average digestion product is the smallest polytetranucleotide. DNase I can catalyze many forms of DNA, such as single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is affected by histones).The optimum pH range is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions, such as Co2+, Mn2+, Zn2+, etc. 5 mM Ca2+ can protect the enzyme from being hydrolyzed. In the presence of Mg2+, the enzyme can recognize and cut any site on any strand of DNA randomly; and in the presence of Mn2+, it can recognize two strands of DNA at the same time and cut at almost the same site to form blunt ends, Or sticky ends with 1-2 nucleotides protruding. DNase I is widely used in the preparation of DNA-free RNA; remove the template DNA after in vitro transcription; prepare DNA-free RNA before RT-PCR and RT-qPCR reactions; combine with DNA polymerase I to perform DNA labeling through nick translocation; DNA fragmentation library construction.

     

    Specifications

    Source
    Recombinant E. coli with DNase I gene.
    Optimum Temperature
    37℃
    Storage Buffer
    10 mM Tris-HCl pH 7.62 mM CaCl250%(v/v) Glycerol.
    Unit Definition
    The amount of enzyme required to increase the absorbance at 260 nm of the reaction solution by 0.001 in 1 minute at 25°C and pH 5.0 using calf thymus DNA as the substrate is defined as one activity unit (Kunitz Unit).(The reaction buffer is: 10 mM Tris-HCl pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 μg plasmid DNA.)


    Shipping and Storage

    Shipped on dry ice. Store at -15°C~ -25°C for one years.



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