Human BMP-4 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of BMP-4 in serum, plasma, and cell culture supernatants. Specific anti-BMP-4 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, BMP-4 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of BMP-4 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).
Bone Morphogenetic Protein 4(BMP4) belongs to the TGF-β superfamily, playing essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
Specification
|
Item Number
|
P162015S / P162015E |
| Specification |
48 T / 96 T |
| Detection Range |
15.63-1000 pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Time |
4.5 hours |
| Sensitivity |
11.2 pg/mL |
| Dilution Linearity |
86 - 127% |
| Recovery Rate |
75 - 126% |
| Intra-assay Variability |
4.0% |
| Inter-assay Variability |
5.0% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162015S |
P162015E |
| P162015-A |
Plate |
2~8℃ |
48 T |
96 T |
| P162015-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162015-C |
Detection Antibody |
2~8℃ |
60 μL |
120 μL |
| P162015-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162015-E |
Sample Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162015-F |
Antibody/Enzyme Dilution Buffer |
2~8℃ |
15 mL |
30 mL |
| P162015-G |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162015-H |
Substrate Solution |
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162015-I |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162015-J |
Plate Sealers |
Room Temperature |
3 pieces |
5 pieces |
Storage
The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After First Use
| Material Name
|
Storage Conditions |
| Plate |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Sample Dilution Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20x Wash Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Antibody/Enzyme Dilution Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure.
|
| Stop Solution |
Can be stored at room temperature. |
| Plate Sealers |
Can be stored at room temperature. |
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